<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sardans, J.</style></author><author><style face="normal" font="default" size="100%">Gargallo-Garriga, A.</style></author><author><style face="normal" font="default" size="100%">Pérez-Trujillo, M.</style></author><author><style face="normal" font="default" size="100%">Parella, T. J.</style></author><author><style face="normal" font="default" size="100%">Seco, R.</style></author><author><style face="normal" font="default" size="100%">Filella, I.</style></author><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metabolic responses of Quercus ilex seedlings to wounding analysed with nuclear magnetic resonance profiling</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Asparagine</style></keyword><keyword><style  face="normal" font="default" size="100%">choline</style></keyword><keyword><style  face="normal" font="default" size="100%">glucose</style></keyword><keyword><style  face="normal" font="default" size="100%">herbivory</style></keyword><keyword><style  face="normal" font="default" size="100%">NMR metabolomics</style></keyword><keyword><style  face="normal" font="default" size="100%">quercitol</style></keyword><keyword><style  face="normal" font="default" size="100%">quinic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">wounding</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/plb.12032</style></url></web-urls></urls><pages><style face="normal" font="default" size="100%">n/a - n/a</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Plants defend themselves against herbivory at several levels. One of these is the synthesis of inducible chemical defences. Using NMR metabolomic techniques, we studied the metabolic changes of plant leaves after a wounding treatment simulating herbivore attack in the Mediterranean sclerophyllous tree Quercus ilex. First, an increase in glucose content was observed in wounded plants. There was also an increase in the content of C-rich secondary metabolites such as quinic acid and quercitol, both related to the shikimic acid pathway and linked to defence against biotic stress. There was also a shift in N-storing amino acids, from leucine and isoleucine to asparagine and choline. The observed higher content of asparagine is related to the higher content of choline through serine that was proved to be the precursor of choline. Choline is a general anti-herbivore and pathogen deterrent. The study shows the rapid metabolic response of Q. ilex in defending its leaves, based on a rapid increase in the production of quinic acid, quercitol and choline. The results also confirm the suitability of 1H NMR-based metabolomic profiling studies to detect global metabolome shifts after wounding stress in tree leaves, and therefore its suitability in ecometabolomic studies.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Copolovici, L. O.</style></author><author><style face="normal" font="default" size="100%">Filella, I.</style></author><author><style face="normal" font="default" size="100%">Llusia, J.</style></author><author><style face="normal" font="default" size="100%">Niinemets, U.</style></author><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">The capacity for thermal protection of photosynthetic electron transport varies for different monoterpenes in Quercus ilex</style></title><secondary-title><style face="normal" font="default" size="100%">PLANT PHYSIOLOGY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">foliar photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">heat stress resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">139</style></volume><pages><style face="normal" font="default" size="100%">485 - 496</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Heat stress resistance of foliar photosynthetic apparatus was investigated in the Mediterranean monoterpene-emitting evergreen sclerophyll species Quercus ilex. Leaf feeding with fosmidomycin, which is a specific inhibitor of the chloroplastic isoprenoid synthesis pathway, essentially stopped monoterpene emission and resulted in the decrease of the optimum temperature of photosynthetic electron transport from approximately 38 degrees C to approximately 30 degrees C. The heat stress resistance was partly restored by fumigation with 4 to 5 nmol mol(-1) air concentrations of monoterpene alpha-pinene but not with fumigations with monoterpene alcohol alpha-terpineol. Analyses of monoterpene physicochemical characteristics demonstrated that alpha-pinene was primarily distributed to leaf gas and lipid phases, while alpha-terpineol was primarily distributed to leaf aqueous phase. Thus, for a common monoterpene uptake rate, alpha-terpineol is less efficient in stabilizing membrane liquid-crystalline structure and as an antioxidant in plant membranes. Furthermore, alpha-terpineol uptake rate ( U) strongly decreased with increasing temperature, while the uptake rates of alpha-pinene increased with increasing temperature, providing a further explanation of the lower efficiency of thermal protection by alpha-terpineol. The temperature-dependent decrease of alpha-terpineol uptake was both due to decreases in stomatal conductance, g(w), and increased volatility of alpha-terpineol at higher temperature that decreased the monoterpene diffusion gradient between the ambient air (F-A) and leaf (F-I; U=g(w)\{[\}F-A - F-I]). Model analyses suggested that alpha-pinene reacted within the leaf at higher temperatures, possibly within the lipid phase, thereby avoiding the decrease in diffusion gradient, F-A-F-I. Thus, these data contribute to the hypothesis of the antioxidative protection of leaf membranes during heat stress by monoterpenes. These data further suggest that fumigation with the relatively low atmospheric concentrations of monoterpenes that are occasionally observed during warm windless days in the Mediterranean canopies may significantly improve the heat tolerance of nonemitting vegetation that grows intermixed with emitting species.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">APSAPSThe following values have no corresponding Zotero field:&lt;br/&gt;pub-location: 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA&lt;br/&gt;publisher: AMER SOC PLANT BIOLOGISTS</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author><author><style face="normal" font="default" size="100%">Filella, I.</style></author><author><style face="normal" font="default" size="100%">Llusia, J.</style></author><author><style face="normal" font="default" size="100%">Siscart, D.</style></author><author><style face="normal" font="default" size="100%">Piñol, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative field study of spring and summer leaf gas exchange and photobiology of the mediterranean trees Quercus ilex and Phillyrea latifolia</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">intrinsic water use efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">net photosynthetic rates</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">photochemical efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">photochemical reflectance index</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic radiation-use-efficiency</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">reflectance</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword><keyword><style  face="normal" font="default" size="100%">summer</style></keyword><keyword><style  face="normal" font="default" size="100%">water index</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1998///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://jxb.oxfordjournals.org/content/49/319/229.abstract</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">49</style></volume><pages><style face="normal" font="default" size="100%">229 - 238</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Summer-induced changes in gas exchange, fluorescence and reflectance were measured on leaves of two co-occurring Mediterranean small trees, Quercus ilex and Phillyrea latifolia, in May, June and July 1996 in Central Catalonia (NE Spain). The humid 1996 summer only produced mild water stress conditions. However, photosynthesis (A) and stomatal conductance (gs) decreased in June and July in both species. In June P. latifolia had higher net photosynthetic rates and lower stomatal conductances than Q. ilex, thus exhibiting higher instantaneous plant water use efficiencies. In agreement with these results, the photo-chemical reflectance index (PRI, calculated as (R570-R531)/(R531+R570)) of P. latifolia was lower, suggesting a possible lower xanthophyll de-epoxidation state. However, P. latifolia had lower ΔF/F′ and therefore a lower electron transport rate (ETR). The behaviour of PRI confirmed previous studies indicating a strong relationship between PRI, ΔF/F′ , and photosynthetic radiation-use efficiency (PRUE). PRI offers a simple, portable means of assessing PRUE with the potential for remote sensing applications. Finally, the possible ecological consequences of these results on the behaviour of the two species studied under the predicted warmer and drier conditions of global change are discussed.</style></abstract><issue><style face="normal" font="default" size="100%">319</style></issue><notes><style face="normal" font="default" size="100%">10.1093/jxb/49.319.22910.1093/jxb/49.319.229</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filella, I.</style></author><author><style face="normal" font="default" size="100%">Llusia, J.</style></author><author><style face="normal" font="default" size="100%">Piñol, J.</style></author><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Leaf gas exchange and ﬂuorescence of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental and Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">hF/F’m</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Pistacia lentiscus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1998///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">39</style></volume><pages><style face="normal" font="default" size="100%">213 - 220</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Saplings of Phillyrea latifolia, Pistacia lentiscus and Quercus ilex were witheld watering for 7 days, followed by reirrigation. Incident photosynthetic photon ﬂux density (PPFD), leaf temperature, net photosynthetic rates, stomatal conductance, and photochemical efﬁciency of the photosystem II (DF:F’m) were measured three times during the day. The watered plants had higher photosynthetic rates, stomatal conductances, DF:F’m and ETR than non-watered plants. However, watered plants were mildly water stressed as shown by low ratio of variable to maximal ﬂuorescence (Fv:Fm) and high non-photochemical ﬂuorescence quenching (qN). Their DF:F%m was low in the morning and increased in the evening, following the variations in PPFD. Watered plants of Q. ilex had lower photosynthetic activity, stomatal conductance and photosynthetic radiation use efﬁciency than Ph. latifolia and P. lentiscus, and, conversely, reached the highest DF:F%m and ETR. This seems to indicate a different relationship between photosynthetic activity and electron transport rate in Q. ilex compared to the other two species. Ph. latifolia and P. lentiscus appeared to be better adapted to severe drought than Q. ilex.</style></abstract></record></records></xml>