<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Simeone, Marco C</style></author><author><style face="normal" font="default" size="100%">Piredda, Roberta</style></author><author><style face="normal" font="default" size="100%">Papini, Alessio</style></author><author><style face="normal" font="default" size="100%">Vessella, Federico</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Application of plastid and nuclear markers to DNA barcoding of Euro-Mediterranean oaks (Quercus, Fagaceae): problems, prospects and phylogenetic implications</style></title><secondary-title><style face="normal" font="default" size="100%">Botanical Journal of the Linnean Society</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cerris</style></keyword><keyword><style  face="normal" font="default" size="100%">ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">regional sampling</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><pages><style face="normal" font="default" size="100%">n/a--n/a</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">DNA barcoding, a species identification system based on sequences from a short, standardized DNA region, has emerged recently as a new tool for taxonomists. We investigated the discriminatory power of a subset of highly variable proposed plant barcoding loci (matK, trnH-psbA, ITS2) in Quercus, a taxonomically complex tree genus of global importance. The research included all currently recognized species and some major variants of the Mediterranean region and Europe (32 taxa) and 17 East Asian and North American species used for comparison. Based on sequence character state, we assigned unique plastid haplotypes to 40.8% of the investigated species; ITS2 increased the resolution up to 87.8% of total taxa. Nevertheless, unsuccessful genetic distance-based discrimination questioned the potential efficiency of correct species identification for future studies. Most species appeared to be nonmonophyletic in parallel phylogenetic tests. Three subgeneric groups were outlined, with different rates of within-group variability and geographical differentiation. Members of one of these groups (corresponding to the Eurasian Group Ilex) were paraphyletic to Group Quercus from the New and Old World and the Eurasian Group Cerris. The data gathered indicate that barcoding markers may help to identify closely related species clusters and contribute to the inference of major diversification and evolutionary patterns in oaks, but the methodology per se appears to be of limited efficacy in defining species limits, unless we make a profound revision of traditional Quercus taxonomic categories. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2013, 00, 000–000.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Petroselli, Andrea</style></author><author><style face="normal" font="default" size="100%">Vessella, Federico</style></author><author><style face="normal" font="default" size="100%">Cavagnuolo, Lucia</style></author><author><style face="normal" font="default" size="100%">Piovesan, Gianluca</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ecological behavior of Quercus suber and Quercus ilex inferred by topographic wetness index (TWI)</style></title><secondary-title><style face="normal" font="default" size="100%">Trees</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Ecological gradients</style></keyword><keyword><style  face="normal" font="default" size="100%">Forest ecology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Terrain analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Topographic wetness index</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">Springer Berlin Heidelberg</style></publisher><volume><style face="normal" font="default" size="100%">27</style></volume><pages><style face="normal" font="default" size="100%">1201-1215</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The ecological behaviors of a network of pure evergreen oak stands (Quercus suber L. and Quercus ilex L.) in the Central-Western Mediterranean Basin were investigated toward climatic and edaphic factors implemented with the application of topographic wetness index (TWI). A Categorical Principal Component Analysis (Catpca) using climatic and soil physico-chemical parameters was performed on 23 cork oak and holm oak pure stands with the aim to understand better the effectiveness of TWI for characterizing soil ecology of the two species. Catpca pointed out that, although cork oak and holm oak are able to growth in similar Mediterranean conditions, they show different behaviors in terms of needs and tolerance to soil water content. TWI confirmed such results at local scale, allowing highlighting some interesting features of the species differential ecology. Although both species confirmed to be drought-tolerant, the heliophilous cork oak revealed to dominate the landscape on wettest soils with high TWI values-indicating the capacity to tolerate stresses due to periods of waterlogging-, while the shade-tolerant holm oak prevails for low-medium TWI values-drier and mesophilous sites. Despite the application of TWI to vegetation science and ecology is relatively recent, results are encouraging and suggest considering this user-friendly and synthetic index in ecological investigations and modeling.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vessella, Federico</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Predicting potential distribution of Quercus suber in Italy based on ecological niche models: Conservation insights and reforestation involvements</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Ecology and Management</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Conservation and reforestation areas</style></keyword><keyword><style  face="normal" font="default" size="100%">Distribution patterns</style></keyword><keyword><style  face="normal" font="default" size="100%">GARP</style></keyword><keyword><style  face="normal" font="default" size="100%">Maxent</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier B.V.</style></publisher><volume><style face="normal" font="default" size="100%">304</style></volume><pages><style face="normal" font="default" size="100%">150-161</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Different statistical techniques have been used to model species potential distribution related to environ- mental variables. This paper provides a comprehensive assessments of GARP and MaxEnt methods, and investigates for the first time the probability of occurrence of cork oak (Quercus suber L.) in Italy based on ecological niche modelling approaches. A detailed distribution of the species was achieved during a 3- year National Project (SuberItalia) and 17 environmental layers were employed to obtain the potential distribution of cork oak. The performance of the models were measured using the receiver operating characteristic (ROC) approach and Cohen’s Kappa statistic. Results achieved by GARP and MaxEnt showed as the drought and the cold stresses are the main factors affecting cork oak occurrence in Italy. Moreover, the accuracy of the obtained prediction maps were compared to a specifically calibrated geo-statistical method at regional scale, pointing out a preliminary geographical assessment of the suitable surfaces to set apart for cork oak forest expansion in Italy, thereby useful to address reforestation and conserva- tion concerns to face the ongoing area reduction of these forests.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Piredda, Roberta</style></author><author><style face="normal" font="default" size="100%">Simeone, Marco C</style></author><author><style face="normal" font="default" size="100%">Attimonelli, Marcella</style></author><author><style face="normal" font="default" size="100%">BELLAROSA, Rosanna</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Prospects of barcoding the Italian wild dendroflora: oaks reveal severe limitations to tracking species identity.</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular ecology resources</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA barcoding</style></keyword><keyword><style  face="normal" font="default" size="100%">forest trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Italy</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">regional sampling</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><volume><style face="normal" font="default" size="100%">11</style></volume><pages><style face="normal" font="default" size="100%">72-83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">DNA barcoding may be particularly important in influencing ecology, economic issues, and the fundamental crisis facing biodiversity as a standardized, species-level identification tool for taxonomy assessment. Trees play important roles in the conservation of many land ecosystems, the wood trade, and the definition of biogeographical processes; nevertheless, peculiar biological, evolutionary and taxonomical features will probably constitute an intriguing challenge to barcoders. We examined whether four marker regions (trnh-psba, rbcL, rpoc1, matK) proposed by the Consortium for the Barcode of Life (CBOL) matched species taxonomy in a preliminary tree biodiversity survey of Italian forested land. Our objective was to provide a test of future in situ applications of DNA barcodes by evaluating the efficacy of species discrimination under the criteria of uniformity of methods and natural co-occurrence of the species in the main forest ecosystems. Fifty-two species were included in a floristic study. We obtained 73% total discrimination success, with trnH-psbA as the best performing marker and oaks as the least responsive plants to the markers used. A further taxon-based study of Quercus (thirty specimens, 12 species) revealed that this genus is refractory to barcoding (0% discrimination success), a probable consequence of low variation rate at the plastid genome level, hybridization, and the incidence of biogeography. We conclude that some species-rich tree genera in small geographical regions may prove exceptionally difficult to barcode. Until more efficient markers are developed, we recommend that improved and diversified sampling (multiple locations of sympatric and co-occurring congenerics) be embraced as a timely and important goal for the precise assessment of haplotype specificity to facilitate the productive application of barcoding in practice.</style></abstract><accession-num><style face="normal" font="default" size="100%">21429102</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author><author><style face="normal" font="default" size="100%">Salis, Antonello</style></author><author><style face="normal" font="default" size="100%">Vessella, Federico</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effectiveness of the Miyawaki method in Mediterranean forest restoration programs</style></title><secondary-title><style face="normal" font="default" size="100%">Landscape and Ecological Engineering</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ecological restoration</style></keyword><keyword><style  face="normal" font="default" size="100%">ecotechnology</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean environment</style></keyword><keyword><style  face="normal" font="default" size="100%">potential natural vegetation</style></keyword><keyword><style  face="normal" font="default" size="100%">reforestation practices comparison</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2010///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.springerlink.com/index/10.1007/s11355-010-0117-0</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">81 - 92</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In the 1980s, Professor Akira Miyawaki introduced a new and innovative reforestation approach in Japan with the challenge to restore indigenous ecosystems, and maintaining global environments, including disaster prevention and carbon dioxide (CO2 ) mitigation. Here, natural vegetation successional stages (from bare soil to mature forest) are practically forced and reproduced, accelerating natural successional times. The Miyawaki method has been applied in the Far East, Malaysia, and South America; results have been very impressive, allowing quick environmental restorations of strongly degraded areas. However, these applications have always been made on sites characterized by high precipitation. The same method has never been used in a Mediterranean context distinguished by summer aridity and risk of desertiﬁcation. A ﬁrst test was carried out by the University of Tuscia, Department of Forest and Environment (DAF), 11 years ago in Sardinia (Italy) on an area where traditional reforestation methods had failed. For an appropriate Miyawaki application on this site, the original method was modiﬁed while maintaining its theoretical principles. Results obtained 2 and 11 years after planting are positive: having compared the traditional reforestation techniques, plant biodiversity using the Miyawaki method appears very high, and the new coenosis (plant community) was able to evolve without further operative support after planting. Therefore, the implementation of supplementary technique along with cost reduction might provide a new and innovative tool to foresters and ecological engineering experts for Mediterranean environmental reforestation program.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">BELLAROSA, Rosanna</style></author><author><style face="normal" font="default" size="100%">Simeone, Marco C.</style></author><author><style face="normal" font="default" size="100%">Papini, Alessio</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp.</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular phylogenetics and evolution</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ITS sequences</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary structures</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/15619447</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">34</style></volume><pages><style face="normal" font="default" size="100%">355 - 370</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nuclear ribosomal DNA sequences encoding the 5.8S RNA and the flanking internal transcribed spacers (ITS1 and ITS2) were used to test the phylogenetic relationships within 12 Italian Quercus taxa (Fagaceae). Hypotheses of sequence orthology are tested by detailed inspection of some basic features of oak ITS sequences (i.e., general patterns of conserved domains, thermodynamic stability and predicted conformation of the secondary structure of transcripts) that also allowed more accurate sequence alignment. Analysis of ITS variation supported three monophyletic groups, corresponding to subg. Cerris, Schlerophyllodrys (=Ilex group sensu Nixon) and Quercus, as proposed by Schwarz [Feddes Rep., Sonderbeih. D, 1-200]. A derivation of the &quot;Cerris group&quot; from the &quot;Ilex group&quot; is suggested, with Q. cerris sister to the rest of the &quot;Cerris group.&quot; Quercus pubescens was found to be sister to the rest of the &quot;Quercus group.&quot; The status of hybrispecies of Q. crenata (Q. cerrisxQ. suber) and Q. morisii (Q. ilexxQ. suber) was evaluated and discussed. Finally, the phylogenetic position of the Italian species in a broader context of the genus is presented. The utility of the ITS marker to assess the molecular systematics of oaks is therefore confirmed. The importance of Italy as a region with a high degree of diversity at the population and genetic level is discussed.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 15619447</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">BELLAROSA, Rosanna</style></author><author><style face="normal" font="default" size="100%">Simeone, Marco C</style></author><author><style face="normal" font="default" size="100%">Papini, Alessio</style></author><author><style face="normal" font="default" size="100%">Schirone, Bartolomeo</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Utility of ITS sequence data for phylogenetic reconstruction of Italian Quercus spp.</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular phylogenetics and evolution</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ITS sequences</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Secondary structures</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><volume><style face="normal" font="default" size="100%">34</style></volume><pages><style face="normal" font="default" size="100%">355-370</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nuclear ribosomal DNA sequences encoding the 5.8S RNA and the flanking internal transcribed spacers (ITS1 and ITS2) were used to test the phylogenetic relationships within 12 Italian Quercus taxa (Fagaceae). Hypotheses of sequence orthology are tested by detailed inspection of some basic features of oak ITS sequences (i.e., general patterns of conserved domains, thermodynamic stability and predicted conformation of the secondary structure of transcripts) that also allowed more accurate sequence alignment. Analysis of ITS variation supported three monophyletic groups, corresponding to subg. Cerris, Schlerophyllodrys (=Ilex group sensu Nixon) and Quercus, as proposed by Schwarz [Feddes Rep., Sonderbeih. D, 1-200]. A derivation of the &quot;Cerris group&quot; from the &quot;Ilex group&quot; is suggested, with Q. cerris sister to the rest of the &quot;Cerris group.&quot; Quercus pubescens was found to be sister to the rest of the &quot;Quercus group.&quot; The status of hybrispecies of Q. crenata (Q. cerrisxQ. suber) and Q. morisii (Q. ilexxQ. suber) was evaluated and discussed. Finally, the phylogenetic position of the Italian species in a broader context of the genus is presented. The utility of the ITS marker to assess the molecular systematics of oaks is therefore confirmed. The importance of Italy as a region with a high degree of diversity at the population and genetic level is discussed.</style></abstract><accession-num><style face="normal" font="default" size="100%">15619447</style></accession-num></record></records></xml>