<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Abril, Nieves</style></author><author><style face="normal" font="default" size="100%">Gion, Jean-Marc</style></author><author><style face="normal" font="default" size="100%">Kerner, René</style></author><author><style face="normal" font="default" size="100%">Müller-Starck, Gerhard</style></author><author><style face="normal" font="default" size="100%">Cerrillo, Rafael M. Navarro</style></author><author><style face="normal" font="default" size="100%">Plomion, Christophe</style></author><author><style face="normal" font="default" size="100%">Renaut, Jenny</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Jorrin-Novo, Jesús V.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteomics research on forest trees, the most recalcitrant and orphan plant species.</style></title><secondary-title><style face="normal" font="default" size="100%">Phytochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Forest tree proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">forest trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Woody plants</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/21353265</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">72</style></volume><pages><style face="normal" font="default" size="100%">1219 - 1242</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The contribution of proteomics to the knowledge of forest tree (the most recalcitrant and almost forgotten plant species) biology is being reviewed and discussed, based on the author's own research work and papers published up to November 2010. This review is organized in four introductory sections starting with the definition of forest trees (1), the description of the environmental and economic importance (2) and its derived current priorities and research lines for breeding and conservation (3) including forest tree genomics (4). These precede the main body of this review: a general overview to proteomics (5) for introducing the forest tree proteomics section (6). Proteomics, defined as scientific discipline or experimental approach, it will be discussed both from a conceptual and methodological point of view, commenting on realities, challenges and limitations. Proteomics research in woody plants is limited to a reduced number of genera, including Pinus, Picea, Populus, Eucalyptus, and Fagus, mainly using first-generation approaches, e.g., those based on two-dimensional electrophoresis coupled to mass spectrometry. This area joins the own limitations of the technique and the difficulty and recalcitrance of the plant species as an experimental system. Furthermore, it contributes to a deeper knowledge of some biological processes, namely growth, development, organogenesis, and responses to stresses, as it is also used in the characterization and cataloguing of natural populations and biodiversity (proteotyping) and in assisting breeding programmes.</style></abstract><issue><style face="normal" font="default" size="100%">10</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Ltd&lt;br/&gt;accession-num: 21353265</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Echevarría-Zomeño, Sira</style></author><author><style face="normal" font="default" size="100%">Abril, Nieves</style></author><author><style face="normal" font="default" size="100%">Ruiz-Laguna, Julia</style></author><author><style face="normal" font="default" size="100%">Jorrín-novo, Jesús</style></author><author><style face="normal" font="default" size="100%">Maldonado-Alconada, Ana M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Simple, rapid and reliable methods to obtain high quality RNA and genomic DNA from Quercus ilex L. leaves suitable for molecular biology studies</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Physiologiae Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">á high-quality rna</style></keyword><keyword><style  face="normal" font="default" size="100%">dna and rna</style></keyword><keyword><style  face="normal" font="default" size="100%">dna and rna extraction</style></keyword><keyword><style  face="normal" font="default" size="100%">extraction á genomic dna</style></keyword><keyword><style  face="normal" font="default" size="100%">genomic dna</style></keyword><keyword><style  face="normal" font="default" size="100%">high-quality rna</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">quercus ilex leaves á</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><volume><style face="normal" font="default" size="100%">34</style></volume><pages><style face="normal" font="default" size="100%">793-805</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Isolation of high-quality RNA and genomic DNA (gDNA) from many samples is a necessary step before accomplishing molecular biology studies. The particular composition of Quercus ilex leaves, specially hard and rich in cell wall material, polyphenolics and secondary metabolites, usually results in preparations contaminated with non-nucleic acid compounds. Although many methods have been developed, each case of study demands a protocol adapted to the speciﬁc plant sample and the pursued research objectives. We have evaluated several protocols to establish the methodology that best suited to our current genetic and molecular studies on Q. ilex. Our priority was to select the simplest methods reducing the plant starting material and the time employed, without compromising yield, quality and integrity of the isolated nucleic acids. Our results point to two protocols based on silicamembrane puriﬁcation, as the most convenient for Q. ilex leaf tissue, and both procedures are greatly improved by adding insoluble polyvinyl polypyrrolidone during the isolation process. The protocols optimized here can be completed at the microfuge scale and allow a researcher to process 48 samples in 1 h, producing high quality preparations suitable for the routinely molecular biology applications with higher efﬁciency than other more labour and time-consuming protocols</style></abstract></record></records></xml>