<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Devi, Sanjenbam Kunjeshwori</style></author><author><style face="normal" font="default" size="100%">Singh, Senjam Sunil</style></author><author><style face="normal" font="default" size="100%">Singh, Sorokhaibam Jibankumar</style></author><author><style face="normal" font="default" size="100%">Rully, Huidrom</style></author><author><style face="normal" font="default" size="100%">Singh, Laishram Rupachandra</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Purification and Characterization of a Magnesium Ion Requiring N-Acetyl-D-glucosamine Specific Lectin from Seeds of Quercus ilex L.</style></title><secondary-title><style face="normal" font="default" size="100%">BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">lectin</style></keyword><keyword><style  face="normal" font="default" size="100%">magnesium ion requiring lectin</style></keyword><keyword><style  face="normal" font="default" size="100%">N-acetyl-D-glucosamine specific lectin</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex L.</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">75</style></volume><pages><style face="normal" font="default" size="100%">1752 - 1757</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A new magnesium ion requiring N-acetyl-D-glucosamine specific lectin QIL was purified to electrophoretic homogeneity from seeds of Quercus ilex L. through successive steps of (i) lectin extraction, (ii) ammonium sulphate (30-50%) fractionation, (iii) diethylaminoethyl (DEAE)-cellulose chromatography, (iv) carboxymethyl (CM)-cellulose chromatography, and (v) Sephadex G-75 chromatography. The lectin, having specific activity of 25,600 hemagglutination units (HAU)/mg of protein, was found to be a monomeric protein with a native molecular weight of 13.2 kDa. N-Acetyl-D-glucosamine was found to exhibit most potent inhibitory action on the lectin activity among all the sugars tested. The lectin was also found to exhibit specificity for human blood groups A, B, and AB. It was converted to the corresponding apo-lectin by ethylenediaminetetraacetic acid (EDTA) treatment followed by buffer dialysis. The apo-lectin exhibited a specific and characteristic requirement for magnesium ions for the expression of its activity.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: JAPAN ACAD SOC CTR BLDG, 2-4-6 YAYOI BUNKYO-KU, TOKYO, 113-0032, JAPAN&lt;br/&gt;publisher: JAPAN SOC BIOSCI BIOTECHN AGROCHEM</style></notes></record></records></xml>