<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Raman spectroscopy a promising technique for investigations of metallothioneins.</style></title><secondary-title><style face="normal" font="default" size="100%">Metallomics : integrated biometal science</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><volume><style face="normal" font="default" size="100%">2</style></volume><pages><style face="normal" font="default" size="100%">246-60</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Metallobiomolecules are highly elaborated coordination complexes, and their fundamental metal-ligand interactions are critical components of metalloprotein folding, assembly, stability, electrochemistry, and catalytic function. Herein, we have described the benefits in using Raman spectroscopy to define the metal-ion binding properties of MTs toward metal ions such as Zn(ii) and Cd(ii). In particular, this vibrational technique can shed light on the secondary structures eventually present in MTs and the ligands involved in metal coordination. The oxidation state of Cys residues and their participation in the metal chelation can be clearly defined, as well as the eventual involvement of His residues. With regards to exogenous metal ligands such as sulfide anions, their presence can be identified by some marker bands whose intensity is linearly correlated with sulfide/metal molar ratio. Finally, Raman can be also an useful tool for providing information on the favourite sites of the radical attack and radical-induced modification in protein folding. In conclusion, many advantages such as the capability of defining local regions in large complexes and detecting several structural features at the same time, the ability in supporting mechanisms, as well as the requirement of low sample amount, make to propose Raman spectroscopy, in coupling with analytical techniques such as atomic emission spectroscopy, gas chromatography, and circular dichroism, as one of the most promising experimental strategies in the research on structure-activity relationships in MTs.</style></abstract><accession-num><style face="normal" font="default" size="100%">21069167</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Domènech, J.</style></author><author><style face="normal" font="default" size="100%">Tinti, Anna</style></author><author><style face="normal" font="default" size="100%">Capdevila, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Structural study of the zinc and cadmium complexes of a type 2 plant (Quercus suber) metallothionein: Insights by vibrational spectroscopy</style></title><secondary-title><style face="normal" font="default" size="100%">Biopolymers</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">IR spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">metal complexes</style></keyword><keyword><style  face="normal" font="default" size="100%">metallothionein</style></keyword><keyword><style  face="normal" font="default" size="100%">Raman spectroscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">sulfur ligand</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://onlinelibrary.wiley.com/doi/10.1002/bip.20729/full</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">86</style></volume><pages><style face="normal" font="default" size="100%">240 - 248</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Zn- and Cd-complexes of Quercus suber metallothionein (QsMT) were obtained by in vivo-synthesis, in order to obtain physiologically representative aggregates, and characterized by spectrometric and spectroscopic methods. The secondary structure elements and the coordination environments of the metal binding sites of the two aggregates were determined, as well as the main metal-containing species formed. The results obtained from the analysis of the Raman and IR spectra reveal that these metal-MT complexes predominantly contain b-sheet elements (about 60%), whereas they lack ahelices. These structural features slightly depend on the divalent metal bound. In particular, Cd II binding to QsMT induces a slight increase of the b-sheet percentage, as well as a decrease in b-turn elements with respect to Zn II binding. Conversely, the in vivo capability of QsMT to inglobe metal and sulﬁde ions is metal-depending. Spectroscopic vibrational data also conﬁrm the presence of sulﬁde ligands in the metal clusters of both Zn- and Cd-QsMT, while the participation of the spacer His residue in metal coordination was only found in Cd-QsMT, in agreement with the CD results. Overall data suggest different coordination environments for Zn II and Cd II ions in QsMT</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Structural study of the zinc and cadmium complexes of a type 2 plant (Quercus suber) metallothionein: Insights by vibrational spectroscopy</style></title><secondary-title><style face="normal" font="default" size="100%">Biopolymers</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><volume><style face="normal" font="default" size="100%">86</style></volume><pages><style face="normal" font="default" size="100%">240-248</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Zn- and Cd-complexes of Quercus suber metallothionein (QsMT) were obtained by in vivo-synthesis, in order to obtain physiologically representative aggregates, and characterized by spectrometric and spectroscopic methods. The secondary structure elements and the coordination environments of the metal binding sites of the two aggregates were determined, as well as the main metal-containing species formed. The results obtained from the analysis of the Raman and IR spectra reveal that these metal-MT complexes predominantly contain b-sheet elements (about 60%), whereas they lack ahelices. These structural features slightly depend on the divalent metal bound. In particular, Cd II binding to QsMT induces a slight increase of the b-sheet percentage, as well as a decrease in b-turn elements with respect to Zn II binding. Conversely, the in vivo capability of QsMT to inglobe metal and sulﬁde ions is metal-depending. Spectroscopic vibrational data also conﬁrm the presence of sulﬁde ligands in the metal clusters of both Zn- and Cd-QsMT, while the participation of the spacer His residue in metal coordination was only found in Cd-QsMT, in agreement with the CD results. Overall data suggest different coordination environments for Zn II and Cd II ions in QsMT</style></abstract></record></records></xml>