<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">A landscape genetics approach reveals ecological-based differentiation in populations of holm oak (Quercus ilex L.) at the northern limit of its range</style></title><secondary-title><style face="normal" font="default" size="100%">Biological Journal of the Linnean Society</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">107</style></volume><pages><style face="normal" font="default" size="100%">458-467</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The holm oak plays a relevant role in the functioning of Mediterranean forests. In the area north of Garda Lake, Italian Prealps, holm oak populations are at the northernmost edge of their distribution. Being peripheral, these populations are of particular interest for ecological, evolutionary and conservation studies. Through an explicit individual-based landscape genetics approach, we addressed the following questions: (1) are levels of genetic variation reduced in these marginal populations compared with central populations?; (2) despite the narrow geographical scale, do individual-based analyses have some power to detect genetic differentiation?; (3) do environmental and/or climatic factors exert a role in shaping patterns of genetic variation and differentiation? Through a Bayesian method, we identified three clusters whose genetic variability can be considered to be of the same order as that recorded in central Quercus ilex populations. Although being geographically very close (&lt; 20 km), the differentiation was statistically significant (P &lt; 0.05) with global F</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Donnarumma, Francesca</style></author><author><style face="normal" font="default" size="100%">Paffetti, Donatella</style></author><author><style face="normal" font="default" size="100%">Stotzky, Guenther</style></author><author><style face="normal" font="default" size="100%">Giannini, Raffaello</style></author><author><style face="normal" font="default" size="100%">Vettori, Cristina</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Potential gene exchange between Bacillus thuringiensis subsp. kurstaki and Bacillus spp. in soil in situ</style></title><secondary-title><style face="normal" font="default" size="100%">Soil Biology and Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacillus mycoides</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacillus spp</style></keyword><keyword><style  face="normal" font="default" size="100%">bacillus thuringiensis subsp</style></keyword><keyword><style  face="normal" font="default" size="100%">Bacillus thuringiensis subsp. kurstaki</style></keyword><keyword><style  face="normal" font="default" size="100%">cry genes</style></keyword><keyword><style  face="normal" font="default" size="100%">Horizontal gene transfer</style></keyword><keyword><style  face="normal" font="default" size="100%">kurstaki</style></keyword><keyword><style  face="normal" font="default" size="100%">Natural soil environment</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2010///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S0038071710001069</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">42</style></volume><pages><style face="normal" font="default" size="100%">1329 - 1337</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The possible transfer of genes from Bacillus thuringiensis subsp. kurstaki (Btk) to indigenous Bacillus spp. was investigated in soil samples from stands of cork oak in Orotelli (Sardinia, Italy) collected 5 years after spraying of the stands with a commercial insecticidal preparation (FORAY 48B) of Btk. Two colonies with a morphology different from that of Btk were isolated and identiﬁed as Bacillus mycoides by morphological and physiological characteristics and by 16S rDNA analysis. Ampliﬁcation by the polymerase chain reaction (PCR) of the DNA of the two isolated B. mycoides colonies with primers used for the identiﬁ- cation of the Btk cry genes showed the presence of a fragment of 238 bp of the cry1Ab9 gene that had a similarity of 100% with the sequence of the cry1Ab9 gene present in GenBank, indicating that the isolates of B. mycoides acquired part of the sequence of this gene from Btk. No cells of Btk or B. mycoides carrying the 238-bp fragment of the cry1Ab9 gene were isolated from samples of unsprayed control soil. However, the isolates of B. mycoides were not able to express the partial Cry1Ab protein. Hybridization with probes for IS231 and the cry1Ab9 gene suggested that the inverted repeated sequence, IS231, was probably involved in the transfer of the 238-bp fragment from Btk to B. mycoides. These results indicate that transfer of genes between introduced Btk and indigenous Bacillus spp. can occur in soil under ﬁeld conditions.</style></abstract><issue><style face="normal" font="default" size="100%">8</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Ltd</style></notes></record></records></xml>