<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sanz, J</style></author><author><style face="normal" font="default" size="100%">González-Fernández, I</style></author><author><style face="normal" font="default" size="100%">Calvete-Sogo, H</style></author><author><style face="normal" font="default" size="100%">Lin, J S</style></author><author><style face="normal" font="default" size="100%">Alonso, R</style></author><author><style face="normal" font="default" size="100%">Muntifering, R</style></author><author><style face="normal" font="default" size="100%">Bermejo, V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Ozone and nitrogen effects on yield and nutritive quality of the annual legume Trifolium cherleri</style></title><secondary-title><style face="normal" font="default" size="100%">Atmospheric Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">annual grasslands</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehesa</style></keyword><keyword><style  face="normal" font="default" size="100%">Forage quality</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone fluxes</style></keyword><keyword><style  face="normal" font="default" size="100%">POD</style></keyword><keyword><style  face="normal" font="default" size="100%">Senescence</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><volume><style face="normal" font="default" size="100%">94</style></volume><pages><style face="normal" font="default" size="100%">765-772</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Two independent experiments were performed in an Open-Top Chamber facility to determine the response of biomass and nutritive quality of the annual legume Trifolium cherleri to increased levels of ozone (O3) and nitrogen (N) deposition, two main drivers of global change. Plants growing in pots were exposed to three O3 treatments: charcoal-filtered air (CFA); non-filtered air, reproducing ambient O3 levels of the site (NFA); and non-filtered air supplemented with 40 nl l−1 (NFA+). Nitrogen was added in biweekly doses to achieve final doses of 5 (N5), 15 (N15) and 30 kg ha−1 (N30), reproducing the N deposition range in the Iberian Peninsula. Ozone negatively affected all the growth-related parameters and increased plant senescent biomass. The pollutant affected subterranean biomass to a greater extent than aerial biomass, resulting in altered aerial/subterranean ratio. Effects in the second experiment followed the same pattern as in the first, but were of lesser magnitude. However, these differences between assays could not be explained adequately by the absorbed O3 fluxes (Phytotoxic Ozone Dose, POD). Concentrations of cell-wall constituents related to nutritive quality increased with the O3 exposure, reducing the Relative Food Value index (RFV) that indicates decreased nutritive quality of the forage. Nitrogen stimulated all growth-related parameters, but increased the aboveground biomass more than the subterranean biomass. No effects of N fertilizer were detected for the nutritive quality parameters. A significant interaction between O3 and N was found in the second experiment. N further enhanced the increase of senescent biomass caused by O3. Results indicate that O3 is a potentially significant environmental stress factor in terms of structure and diversity of Mediterranean pastures.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sanz, J</style></author><author><style face="normal" font="default" size="100%">Bermejo, V</style></author><author><style face="normal" font="default" size="100%">Muntifering, R</style></author><author><style face="normal" font="default" size="100%">González-Fernández, I</style></author><author><style face="normal" font="default" size="100%">Gimeno, B S</style></author><author><style face="normal" font="default" size="100%">Elvira, S</style></author><author><style face="normal" font="default" size="100%">Alonso, R</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Plant phenology, growth and nutritive quality of Briza maxima: responses induced by enhanced ozone atmospheric levels and nitrogen enrichment.</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental pollution (Barking, Essex : 1987)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Dehesa annual grasslands</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitrogen. Ozone</style></keyword><keyword><style  face="normal" font="default" size="100%">Nutritive quality</style></keyword><keyword><style  face="normal" font="default" size="100%">yield</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier Ltd</style></publisher><volume><style face="normal" font="default" size="100%">159</style></volume><pages><style face="normal" font="default" size="100%">423-430</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An assessment of the effects of tropospheric ozone (O(3)) levels and substrate nitrogen (N) supplementation, singly and in combination, on phenology, growth and nutritive quality of Briza maxima was carried out. Two serial experiments were developed in Open-Top Chambers (OTC) using three O(3) and three N levels. Increased O(3) exposure did not affect the biomass-related parameters, but enhanced senescence, increased fiber foliar content (especially lignin concentration) and reduced plant life span; these effects were related to senescence acceleration induced by the pollutant. Added N increased plant biomass production and improved nutritive quality by decreasing foliar fiber concentration. Interestingly, the effects of N supplementation depended on meteorological conditions and plant physiological activity. N supplementation counteracted the O(3)-induced senescence but did not modify the effects on nutritive quality. Nutritive quality and phenology should be considered in new definitions of the O(3) limits for the protection of herbaceous vegetation.</style></abstract><accession-num><style face="normal" font="default" size="100%">21111519</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alonso, R</style></author><author><style face="normal" font="default" size="100%">Bermejo, V</style></author><author><style face="normal" font="default" size="100%">Sanz, J</style></author><author><style face="normal" font="default" size="100%">Valls, B</style></author><author><style face="normal" font="default" size="100%">Elvira, S</style></author><author><style face="normal" font="default" size="100%">Gimeno, B S</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Stomatal conductance of semi-natural Mediterranean grasslands: implications for the development of ozone critical levels.</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental pollution (Barking, Essex : 1987)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Critical levels</style></keyword><keyword><style  face="normal" font="default" size="100%">Grasslands</style></keyword><keyword><style  face="normal" font="default" size="100%">Ozone</style></keyword><keyword><style  face="normal" font="default" size="100%">Semi-natural vegetation</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><volume><style face="normal" font="default" size="100%">146</style></volume><pages><style face="normal" font="default" size="100%">692-698</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Intra-genus and intra-specific variation and the influence of nitrogen enrichment on net assimilation and stomatal conductance of some annual Trifolium species of Mediterranean dehesa grasslands were assessed under experimental conditions. Also gas exchange rates were compared between some Leguminosae and Poaceae species growing in the field in a dehesa ecosystem in central Spain. The results showed that the previously reported different O3 sensitivity of some Trifolium species growing in pots does not seem to be related to different maximum g(s) values. In addition, no clear differences on gas exchange rates could be attributed to Leguminosae and Poaceae families growing in the field, with intra-genus variation being more important than differences found between families. Further studies are needed to increase the database for developing a flux-based approach for setting O3 critical levels for semi-natural Mediterranean species.</style></abstract><accession-num><style face="normal" font="default" size="100%">16895740</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gimeno, B S</style></author><author><style face="normal" font="default" size="100%">Bermejo, V</style></author><author><style face="normal" font="default" size="100%">Sanz, J</style></author><author><style face="normal" font="default" size="100%">de la Torre, D</style></author><author><style face="normal" font="default" size="100%">Gil, J M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Assessment of the effects of ozone exposure and plant competition on the reproductive ability of three therophytic clover species from Iberian pastures</style></title><secondary-title><style face="normal" font="default" size="100%">Atmospheric Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Critical levels</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehesa</style></keyword><keyword><style  face="normal" font="default" size="100%">flower biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean grasslands</style></keyword><keyword><style  face="normal" font="default" size="100%">seed production</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><volume><style face="normal" font="default" size="100%">38</style></volume><pages><style face="normal" font="default" size="100%">2295-2303</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Ozone (O3 ) phytototoxicity has been reported on a wide range of crops and wild Central European plant species, however no information has been provided regarding the sensitivity of plant species from dehesa Mediterranean therophytic grasslands in spite of their great plant species richness and the high O3 levels that are recorded in this area. A study was carried out in open-top chambers (OTCs) to assess the effects of O3 and competition on the reproductive ability of three clover species: Trifolium cherleri, Trifolium subterraneum and Trifolium striatum. A phytometer approach was followed, therefore plants of these species were grown in mesoscosms composed of monocultures of four plants of each species, of three plants of each species competing against a Briza maxima individual or of a single plant of each clover species competing with three B. maxima plants. Three O3 treatments were adopted: charcoal ﬁltered air (CFA), non-ﬁltered air (NFA) and non-ﬁltered air supplemented with 40 nl l 1 of O3 (NFA+). The different mesocosms were exposed to the different O3 treatments for 45 days and then they remained in the open. Ozone exposure caused reductions in the ﬂower biomass of the three clover species assessed. In the case of T. cherleri and T. subterraneum this effect was found following their exposure to the different O3 treatments during their vegetative period. An attenuation of these effects was found when the plants remained in the open. Ozone-induced detrimental effects on the seed output of T. striatum were also observed. The ﬂower biomass of the clover plants grown in monocultures was greater than when competing with one or three B. maxima individuals. An increased ﬂower biomass was found in the CFA monoculture mesocosms of T. cherleri when compared with the remaining mesocosms, once the plants were exposed in the open for 60 days. The implications of these effects on the performance of dehesa acid grasslands and for the definition of O3 critical levels is discussed</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gimeno, B S</style></author><author><style face="normal" font="default" size="100%">Bermejo, V</style></author><author><style face="normal" font="default" size="100%">Sanz, J</style></author><author><style face="normal" font="default" size="100%">de la Torre, D</style></author><author><style face="normal" font="default" size="100%">Elvira, S</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Growth response to ozone of annual species from Mediterranean pastures.</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental pollution (Barking, Essex : 1987)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Critical levels</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehesa</style></keyword><keyword><style  face="normal" font="default" size="100%">Relative growth rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Screening</style></keyword><keyword><style  face="normal" font="default" size="100%">Therophytes</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><volume><style face="normal" font="default" size="100%">132</style></volume><pages><style face="normal" font="default" size="100%">297-306</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Ozone (O3) phytotoxicity has been reported on a wide range of plant species. However, scarce information has been provided regarding the sensitivity of semi-natural grassland species, especially those from dehesa Mediterranean grasslands, in spite of their great biological diversity and the high O3 levels recorded in the region. A screening study was carried out in open-top chambers (OTCs) to assess the O3-sensitivity of representative therophytes of these ecosystems based on the response of selected growth-related parameters. Three O3 treatments and 3 OTCs per treatment were used. Legume species were very sensitive to O3, because 78% of the tested species showed detrimental effects on their total biomass relative growth rate (RGR) following their exposure to O3. The Trifolium genus was particularly sensitive showing O3-induced adverse effects on most of the assessed parameters. Gramineae plants were less sensitive than Leguminosae species because detrimental effects on total biomass RGR were only observed in 14% of the assessed species. No relationship was found between relative growth rates when growing in clean air and O3 susceptibility. The implications of these effects on the performance of dehesa acidic grasslands and on the definition of ozone critical levels for the protection of semi-natural vegetation are discussed.</style></abstract><accession-num><style face="normal" font="default" size="100%">15312942</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Almendros, G</style></author><author><style face="normal" font="default" size="100%">Sanz, J</style></author><author><style face="normal" font="default" size="100%">VELASCO, F</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Signatures of lipid assemblages in soils under continental Mediterranean forests</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Soil Science</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">GC-MS</style></keyword><keyword><style  face="normal" font="default" size="100%">Lipids</style></keyword><keyword><style  face="normal" font="default" size="100%">Lipids assemblage (voyant)</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean forest soils</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1996</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">47</style></volume><pages><style face="normal" font="default" size="100%">183-196</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The analysis of free lipids in 12 soils from three representative types of Mediterranean forest has been carried out in an attempt to describe diagnostic molecules reflecting differences between the ecosystems and the intensity of the soil organic matter turnover. The study centred on the analysis by gas chromatography–mass spectrometry of the soil compounds extracted with petroleum ether from soils in central Spain, developed under monospecific formations of stone pine (Pinus pined), evergreen oak (Quercus rotundifolia) and Spanish juniper (Juniperus thurifera), the lipid extracts from their leaves also being analysed as reference material. The comparison between the distribution patterns of alkanes and fatty acids in plant lipids and the corresponding soil lipids was used to assess the extent to which the former accumulate in soil or are substituted by other biogenic or diagenetic homologues. In general, the alkane patterns showed the greatest variation in soils under oak, and the differences between lipid patterns in plant and soil were greatest in the juniper forests. As indicators of the vegetation type, the soil fatty acids had little value. Up to 60 major cyclic compounds were identified, including mainly di- and sesquiterpenes, in addition to some monoterpenes and nonterpenic naphthalenes and decalins. Of these major constituents, 33 compounds were found in soil but were not present in plant extracts, and 18 compounds were identified in plants but were not in the soils. The results suggest an arrangement of the soil samples based on the composition of the signature lipid assemblages.</style></abstract></record></records></xml>