<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Álvarez, R</style></author><author><style face="normal" font="default" size="100%">Alonso, P</style></author><author><style face="normal" font="default" size="100%">Cortizo, M</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">HERNÁNDEZ, I</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author><author><style face="normal" font="default" size="100%">Ordás, R J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of selected mature cork oak (Quercus suber L.) trees.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant cell reports</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agrobacterium</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Tree genetic transformation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year></dates><volume><style face="normal" font="default" size="100%">23</style></volume><pages><style face="normal" font="default" size="100%">218-223</style></pages><isbn><style face="normal" font="default" size="100%">3498510479</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A transformation system for selected mature cork oak (Quercus suber L.) trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses were inoculated with A. tumefaciens strains EHA105, LBA4404 or AGL1 harbouring the plasmid pBINUbiGUSint [carrying the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) genes]. The highest transformation efficiency (4%) was obtained when freshly isolated explants were inoculated with A. tumefaciens strain AGL1. Evidence of stable transgene integration was obtained by PCR for the nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos were germinated and successfully transferred to soil.</style></abstract><accession-num><style face="normal" font="default" size="100%">15185122</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hornero, J</style></author><author><style face="normal" font="default" size="100%">Gallego, F J</style></author><author><style face="normal" font="default" size="100%">Martinez, I</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Testing the conservation of Quercus spp. microsatellites in the cork oak, Q-suber L.</style></title><secondary-title><style face="normal" font="default" size="100%">SILVAE GENETICA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">J D SAUERLANDERS VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">FINKENHOFSTRASSE 21, D-60322 FRANKFURT, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">50</style></volume><pages><style face="normal" font="default" size="100%">162-167</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The transferability of microsatellite (SSR) loci from Quercus spp. to cork oak (Quercus suber L.) was investigated. Semi-automated analysis of fluorescently-labelled PCR fragments was used to test 24 primer sets developed for Q. myrsinifolia BLUME, Q. petraea (MAT7.) LIEB. and Q. robur L. in 41 cork oak trees from four stands covering the main area of distribution of the species in Spain. Successful cross-species events occurred for 13 loci (54%). Two of them were monomorphic and another two appeared as multilocus. High levels of genetic variability were detected both for the number of alleles, 62 (7.5 per polymorphic locus, with a maximum number of 19 in locus ssrQpZAG110) and for the expected heterozygosity (mean H-E = 0.648). These results were much higher than those previously reported by other authors using allozyme loci. The usefulness of the. SSR loci successfully amplified for studies on population genetics of cork oak is discussed.</style></abstract></record></records></xml>