<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Burgarella, C</style></author><author><style face="normal" font="default" size="100%">Lorenzo, Z</style></author><author><style face="normal" font="default" size="100%">Jabbour-Zahab, R</style></author><author><style face="normal" font="default" size="100%">Lumaret, R</style></author><author><style face="normal" font="default" size="100%">Guichoux, E</style></author><author><style face="normal" font="default" size="100%">Petit, R J</style></author><author><style face="normal" font="default" size="100%">Soto, Á</style></author><author><style face="normal" font="default" size="100%">Gil, L</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection of hybrids in nature: application to oaks (Quercus suber and Q. ilex).</style></title><secondary-title><style face="normal" font="default" size="100%">Heredity</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">clustering analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">introgressive hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword><keyword><style  face="normal" font="default" size="100%">Q. ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Simulation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">102</style></volume><pages><style face="normal" font="default" size="100%">442-452</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Powerful and accurate detection of first-generation (F1) hybrids and backcrosses in nature is needed to achieve a better understanding of the function and dynamics of introgression. To document the frequency of ongoing interspecific gene exchange between two Mediterranean evergreen oaks, the cork oak (Quercus suber) and the holm oak (Q. ilex), we analyzed 1487 individuals originating from across the range of the two species using eight microsatellite loci and two Bayesian clustering approaches (implemented in the programs STRUCTURE and NEWHYBRIDS). Simulated data were used to assess the differences between the two clustering methods and to back up the choice of the threshold value for the posterior probability to discriminate admixed from pure individuals. We found that the use of STRUCTURE resulted in the highest power to detect hybrids, whereas NEWHYBRIDS provided the highest accuracy. Irrespective of the approach, the two species were clearly distinguished as independent genetic entities without any prior information. In contrast with previous reports, we found no evidence for unidirectional introgression. The overall hybridization rate was very low (&lt;2% of introgressed individuals). Only two individuals were identified as F1 hybrids and five as early backcrosses. This work shows that the combined application of the two complementary Bayesian approaches and their systematic validation with simulations, fit for the case at hand, helps gain resolution in the identification of admixed individuals.</style></abstract><accession-num><style face="normal" font="default" size="100%">19240752</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Deguilloux, M.-F.</style></author><author><style face="normal" font="default" size="100%">Dumolin-Lapègue, S</style></author><author><style face="normal" font="default" size="100%">Gielly, L</style></author><author><style face="normal" font="default" size="100%">Grivet, D</style></author><author><style face="normal" font="default" size="100%">Petit, R J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A set of primers for the amplification of chloroplast microsatellites in Quercus</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Ecology Notes</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">chloroplast genome</style></keyword><keyword><style  face="normal" font="default" size="100%">cpSSR</style></keyword><keyword><style  face="normal" font="default" size="100%">forest tree</style></keyword><keyword><style  face="normal" font="default" size="100%">oak</style></keyword><keyword><style  face="normal" font="default" size="100%">population genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">simple sequence repeat</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing, Ltd</style></publisher><volume><style face="normal" font="default" size="100%">3</style></volume><pages><style face="normal" font="default" size="100%">24-27</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The increase in demand for the certification of oak seed lots, as well as control of the geographical origin of oak wood, has led us to develop powerful genetic markers permitting us to discriminate among provenance regions. With the aim of detecting new chloroplast variants, we have identified 17 potential cpSSRs motifs from available oak sequences and tested their variability among French oak populations. Six loci were polymorphic at the intraspecific level in Quercus petraea and Q. robur. Moreover, conservation of the primer pairs was checked on a set of 21 forest tree species and they were all shown to work well on several Quercus species, and even within Fagacaea.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Belahbib, N</style></author><author><style face="normal" font="default" size="100%">Pemonge, M.-H. H</style></author><author><style face="normal" font="default" size="100%">Ouassou, A</style></author><author><style face="normal" font="default" size="100%">Sbay, H</style></author><author><style face="normal" font="default" size="100%">Kremer, A</style></author><author><style face="normal" font="default" size="100%">Petit, R J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Frequent cytoplasmic exchanges between oak species that are not closely related: Quercus suber and Q. ilex in Morocco</style></title><secondary-title><style face="normal" font="default" size="100%">Molecular Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chloroplast</style></keyword><keyword><style  face="normal" font="default" size="100%">Chloroplast: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">cpDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Evolution</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">geographical structure</style></keyword><keyword><style  face="normal" font="default" size="100%">Haplotypes</style></keyword><keyword><style  face="normal" font="default" size="100%">Hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">introgression</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondrial: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Models</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">Morocco</style></keyword><keyword><style  face="normal" font="default" size="100%">mtDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">PCR–RFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Science Ltd</style></publisher><volume><style face="normal" font="default" size="100%">10</style></volume><pages><style face="normal" font="default" size="100%">2003-2012</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Chloroplast (cp) and mitochondrial (mt) DNA variation were studied in 97 populations of cork oak (Quercus suber) in Morocco; in 31 of these populations, holm oak (Quercus ilex), a clearly distinct species, also occurred and was compared with Q. suber. Three cpDNA and one mtDNA primer pairs were used in the survey, each in combination with one restriction enzyme. Six haplotypes belonging to two very divergent lineages were detected; one lineage predominates in each species, and is probably ancestral, as inferred from comparisons with other oak species. In the mixed-species populations, cytoplasmic genomes were frequently shared across species, as indicated by an introgression ratio of 0.63. This index is a new measure of the propensity of species to share locally genetic markers, varying from zero (complete differentiation) to one (no differentiation). By contrast, more closely related deciduous oak species (Q. robur, Q. petraea and Q. pubescens) have introgression ratios varying from 0.82 to 0.97. The introgression events appear to have been more frequent in the direction Q. ilex (female) × Q. suber (male), a finding which seems attributable to the flowering phenology of these two species. This asymmetry may have favoured immigration of Q. suber beyond its main range, in regions already colonized by Q. ilex. There, rare hybridization and further introgression through long distance pollen flow have established populations that are morphologically indistinguishable from Q. suber but that have cytoplasmic genomes originating from the local Q. ilex populations.</style></abstract><accession-num><style face="normal" font="default" size="100%">11555243</style></accession-num></record></records></xml>