<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>7</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Romero-Rodríguez, M.Cristina</style></author><author><style face="normal" font="default" size="100%">Maldonado-Alconada, AnaM.</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Jorrin-Novo, JesusV.</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author><author><style face="normal" font="default" size="100%">Komatsu, Setsuko</style></author><author><style face="normal" font="default" size="100%">Weckwerth, Wolfram</style></author><author><style face="normal" font="default" size="100%">Wienkoop, Stefanie</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Back to Osborne. Sequential Protein Extraction and LC-MS Analysis for the Characterization of the Holm Oak Seed Proteome</style></title><secondary-title><style face="normal" font="default" size="100%">Plant Proteomics SE - 27</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Holm oak proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Osborne</style></keyword><keyword><style  face="normal" font="default" size="100%">Seed proteomics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><volume><style face="normal" font="default" size="100%">1072</style></volume><pages><style face="normal" font="default" size="100%">379-389</style></pages><isbn><style face="normal" font="default" size="100%">978-1-62703-630-6</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">It is impossible to capture in just one experiment all or most of the total set of protein species that constitute the cell’s proteome. Thus, according to our results, and even considering that they depend on the experimental system carried out (plant, yeast, fungi, or bacteria), the best protein extraction protocol yielded less than 20 % of the total amount of proteins, as determined by the Kjeldahl method. For this reason, protein cataloguing and the whole proteome characterization require the use of firstly, fractionation techniques at the cellular, subcellular, protein, or peptide level, and secondly, the use of complementary approaches. Within our current research on Holm oak (Quercus ilex subsp. ballota), we aim to characterize its seed proteome. For that we have optimized an experimental workflow in which the Osborne sequential protein extraction (Osborne, Science 28:417–427, 1908) is combined with downstream electrophoretic protein separation or shotgun MS analysis. In general, it can be used to study any plant seed, as well as to investigate on seed maturation and germination, genotype characterization, allergens identification, food traceability, and substantial equivalence, among others.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valero Galván, José</style></author><author><style face="normal" font="default" size="100%">González Fernandez, Raquel</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Rafael Cerrillo, Ma.Navarro</style></author><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author></authors><secondary-authors><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author><author><style face="normal" font="default" size="100%">Komatsu, Setsuko</style></author><author><style face="normal" font="default" size="100%">Weckwerth, Wolfram</style></author><author><style face="normal" font="default" size="100%">Wienkoop, Stefanie</style></author></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Proteotyping of Holm Oak (Quercus ilex subsp. ballota) Provenances Through Proteomic Analysis of Acorn Flour</style></title><secondary-title><style face="normal" font="default" size="100%">Methods in Molecular Biology Plant Proteomics SE - 49</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Holm oak proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant biodiversity</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant proteotyping</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2014</style></year></dates><publisher><style face="normal" font="default" size="100%">Humana Press</style></publisher><volume><style face="normal" font="default" size="100%">1072</style></volume><pages><style face="normal" font="default" size="100%">709-723</style></pages><isbn><style face="normal" font="default" size="100%">978-1-62703-630-6</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Proteomics has become a powerful tool to characterize biodiversity and natural variability in plant species, as well as to catalogue and establish phylogenetic relationships and distances among populations, provenances or ecotypes. In this chapter, we describe the standard proteomics workflow that we currently use in cataloguing Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) populations. Proteins are extracted from acorn flour or pollen by TCA/acetone or TCA/acetone-phenol methods, resolved by one- or two-dimensional gel electrophoresis, and gel images are captured and analyzed by appropriate software and statistical packages. Quantitative or qualitative variable bands or spots are subjected to MS analysis in order to identify them and correlate differences in the protein profile with the phenotypes or environmental conditions.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valero-Galvàn, José</style></author><author><style face="normal" font="default" size="100%">González-Fernández, Raquel</style></author><author><style face="normal" font="default" size="100%">Navarro-Cerrillo, Rafael Maria</style></author><author><style face="normal" font="default" size="100%">Gil-Pelegrín, Eustaquio</style></author><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Proteome Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">Chlorophyll ﬂuorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">drought stress in Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">water potential</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">5110-5123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almer??a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ?12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.</style></abstract><accession-num><style face="normal" font="default" size="100%">24088139</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></research-notes></record></records></xml>