<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alves, Sofia</style></author><author><style face="normal" font="default" size="100%">Ribeiro, Teresa</style></author><author><style face="normal" font="default" size="100%">Inácio, Vera</style></author><author><style face="normal" font="default" size="100%">Rocheta, Margarida</style></author><author><style face="normal" font="default" size="100%">Morais-Cecílio, Leonor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genomic organization and dynamics of repetitive DNA sequences in representatives of three Fagaceae genera</style></title><secondary-title><style face="normal" font="default" size="100%">Genome</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Castanea</style></keyword><keyword><style  face="normal" font="default" size="100%">euchromatin</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">retroelements</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1139/g2012-020</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">55</style></volume><pages><style face="normal" font="default" size="100%">348 - 359</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Oaks, chestnuts, and beeches are economically important species of the Fagaceae. To understand the relationship between these members of this family, a deep knowledge of their genome composition and organization is needed. In this work, we have isolated and characterized several AFLP fragments obtained from Quercus rotundifolia Lam. through homology searches in available databases. Genomic polymorphisms involving some of these sequences were evaluated in two species of Quercus, one of Castanea, and one of Fagus with specific primers. Comparative FISH analysis with generated sequences was performed in interphase nuclei of the four species, and the co-immunolocalization of 5-methylcytosine was also studied. Some of the sequences isolated proved to be genus-specific, while others were present in all the genera. Retroelements, either gypsy-like of the Tat/Athila clade or copia-like, are well represented, and most are dispersed in euchromatic regions of these species with no DNA methylation associated, pointing to an interspersed arrangement of these retroelements with potential gene-rich regions. A particular gypsy-sequence is dispersed in oaks and chestnut nuclei, but its confinement to chromocenters in beech evidences genome restructuring events during evolution of Fagaceae. Several sequences generated in this study proved to be good tools to comparatively study Fagaceae genome organization.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">doi: 10.1139/g2012-020doi: 10.1139/g2012-020The following values have no corresponding Zotero field:&lt;br/&gt;publisher: NRC Research Press</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">de Heredia, U. L.</style></author><author><style face="normal" font="default" size="100%">JIMÉNEZ, P.</style></author><author><style face="normal" font="default" size="100%">Collada, Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multi-marker phylogeny of three evergreen oaks reveals vicariant patterns in the Western Mediterranean</style></title><secondary-title><style face="normal" font="default" size="100%">Taxon</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">cpDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">ITS</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">vicariance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2007///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ingentaconnect.com/content/iapt/tax/2007/00000056/00000004/art00016</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">56</style></volume><pages><style face="normal" font="default" size="100%">1209 - 1220</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nuclear (AFLPs and ITSs) and chloroplast DNA markers (cpDNA) were analysed in Quercus suber L., Q. ilex L. and Q. coccifera L. in order to detect vicariant patterns in the Western Mediterranean Basin populations. Clustering methods, i.e., parsimony, minimum spanning network, UPGMA and PCO, were used to obtain robust phylogenies. The Mantel test was used to check the geographic structure of the populations. Nuclear markers’ based phylogenies showed high bootstrap values at basal nodes of clusters indicative of species. Within species, Q. suber and Q. ilex showed three and two groups respectively with strong geographic structure for cytoplasmic and nuclear markers. In contrast, Q. coccifera had overlapping cpDNA lineages and nuclear markers did not show any group with strong geographic structure. Taxonomic assignation of the species was well-supported by our data. Ongoing allopatric speciation was inferred for Q. suber and Q. ilex derived from Pliocene vicariant events. Quercus coccifera showed a more homogeneous genetic pattern in the Western Mediterranean.</style></abstract><issue><style face="normal" font="default" size="100%">November</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">de Heredia, U L</style></author><author><style face="normal" font="default" size="100%">Jimenez, P</style></author><author><style face="normal" font="default" size="100%">Collada, Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Multi-marker phylogeny of three evergreen oaks reveals vicariant patterns in the Western Mediterranean</style></title><secondary-title><style face="normal" font="default" size="100%">Taxon</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">cpDNA</style></keyword><keyword><style  face="normal" font="default" size="100%">ITS</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">vicariance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><volume><style face="normal" font="default" size="100%">56</style></volume><pages><style face="normal" font="default" size="100%">1209-1220</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Nuclear (AFLPs and ITSs) and chloroplast DNA markers (cpDNA) were analysed in Quercus suber L., Q. ilex L. and Q. coccifera L. in order to detect vicariant patterns in the Western Mediterranean Basin populations. Clustering methods, i.e., parsimony, minimum spanning network, UPGMA and PCO, were used to obtain robust phylogenies. The Mantel test was used to check the geographic structure of the populations. Nuclear markers’ based phylogenies showed high bootstrap values at basal nodes of clusters indicative of species. Within species, Q. suber and Q. ilex showed three and two groups respectively with strong geographic structure for cytoplasmic and nuclear markers. In contrast, Q. coccifera had overlapping cpDNA lineages and nuclear markers did not show any group with strong geographic structure. Taxonomic assignation of the species was well-supported by our data. Ongoing allopatric speciation was inferred for Q. suber and Q. ilex derived from Pliocene vicariant events. Quercus coccifera showed a more homogeneous genetic pattern in the Western Mediterranean.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Cooke, D. E. L.</style></author><author><style face="normal" font="default" size="100%">Jung, T.</style></author><author><style face="normal" font="default" size="100%">Williams, N. a</style></author><author><style face="normal" font="default" size="100%">Schubert, R.</style></author><author><style face="normal" font="default" size="100%">Osswald, W.</style></author><author><style face="normal" font="default" size="100%">Duncan, J. M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic diversity of European populations of the oak fine-root pathogen Phytophthora quercina</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">decline</style></keyword><keyword><style  face="normal" font="default" size="100%">diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">pathogen (voyant)</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytophthora</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1111/j.1439-0329.2004.00384.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">35</style></volume><pages><style face="normal" font="default" size="100%">57 - 70</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The recently discovered oak-specific fine root plant pathogen Phytophthora quercina is a significant factor in the current phase of European oak decline but its origins and ecology are poorly understood. A genome-wide analysis of 260 amplified fragment length polymorphism (AFLP) markers was used to examine the genetic diversity of 72 isolates from five oak species at 28 sites in Germany (particularly Bavaria), Italy, France, Hungary and the UK. Within-site diversity was examined at 16 sites. The limited genetic diversity (within and between sites) and lack of genetic substructuring according to geographic origin or host species suggest the rapid spread of a relatively recently introduced species. Two subgroups were distinguished and these may reflect an initial introduction of isolates of two different genetic backgrounds. The relatively low genetic diversity is probably because of the predominantly inbreeding (homothallic) nature of P. quercina. However, evidence of limited intra-site diversity, temporal variation and the lack of clonality within the European population suggest that some diversity is being maintained by occasional outcrossing and turnover of a reservoir of long-lived soil-borne oospore (sexually derived) inoculum</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Cooke, D E L</style></author><author><style face="normal" font="default" size="100%">Jung, T</style></author><author><style face="normal" font="default" size="100%">Williams, N a.</style></author><author><style face="normal" font="default" size="100%">Schubert, R</style></author><author><style face="normal" font="default" size="100%">Osswald, W</style></author><author><style face="normal" font="default" size="100%">Duncan, J M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic diversity of European populations of the oak fine-root pathogen Phytophthora quercina</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Pathology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">decline</style></keyword><keyword><style  face="normal" font="default" size="100%">diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">pathogen (voyant)</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytophthora</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year></dates><volume><style face="normal" font="default" size="100%">35</style></volume><pages><style face="normal" font="default" size="100%">57-70</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The recently discovered oak-specific fine root plant pathogen Phytophthora quercina is a significant factor in the current phase of European oak decline but its origins and ecology are poorly understood. A genome-wide analysis of 260 amplified fragment length polymorphism (AFLP) markers was used to examine the genetic diversity of 72 isolates from five oak species at 28 sites in Germany (particularly Bavaria), Italy, France, Hungary and the UK. Within-site diversity was examined at 16 sites. The limited genetic diversity (within and between sites) and lack of genetic substructuring according to geographic origin or host species suggest the rapid spread of a relatively recently introduced species. Two subgroups were distinguished and these may reflect an initial introduction of isolates of two different genetic backgrounds. The relatively low genetic diversity is probably because of the predominantly inbreeding (homothallic) nature of P. quercina. However, evidence of limited intra-site diversity, temporal variation and the lack of clonality within the European population suggest that some diversity is being maintained by occasional outcrossing and turnover of a reservoir of long-lived soil-borne oospore (sexually derived) inoculum</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hornero, J</style></author><author><style face="normal" font="default" size="100%">Martinez, I</style></author><author><style face="normal" font="default" size="100%">CELESTINO, C</style></author><author><style face="normal" font="default" size="100%">Gallego, F J</style></author><author><style face="normal" font="default" size="100%">Torres, V</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">EARLY CHECKING OF GENETIC STABILITY OF CORK OAK SOMATIC EMBRYOS BY AFLP ANALYSIS.</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Plant Sciences</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA markers</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">somaclonal variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">University of Chicago Press</style></publisher><volume><style face="normal" font="default" size="100%">162</style></volume><pages><style face="normal" font="default" size="100%">827</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Early checking of the genetic stability, of tissue culture-derived plants is necessary to obtain all the potential benefits of clonal forestry. Previous work in Quercus suber L. using random amplified polymorphic DNA (RAPD) markers showed no genetic variation among somatic embryos within embryogenic lines of zygotic origin. Genetic fingerprinting based on amplified fragment length polymorphisms (AFLP) allows the direct analysis of variation at the entire DNA level by generating more reproducible markers than RAPDs. To confirm the absence of genetic variation within six embryogenic lines of zygotic origin, six primer pairs were selected out of 48 combinations of primers for revealing up to 512 AFLP markers, 301 of them (58.8%) being polymorphic. The mean number of markers per genotype was 375. Again differences were recorded among embryogenic lines, even between those that arose from half-sib zygotic embryos, but no variation was observed among somatic embryos within embryogenic lines. To check variation in embryogenic lines raised from non-embryonic tissues, somatic embryogenesis was induced in expanding leaves collected from sprouts originating in three mature cork oak. DNA was extracted from leaves and from somatic embryos derived from each tree, and three primer pairs showed 165, 110, and 108 markers per genotype/tree, respectively. In one tree, AFLP patterns generated from leaves and somatic embryos were identical, but variation was detected in samples from the other two trees. Although the level of genetic variation detected in these lines (5.6% and 7.3% of polymorphism, respectively) is lower than that recorded for half sibs of cork oak (25%–31%), its influence on phenotypic variation needs further assessment. [ABSTRACT FROM AUTHOR]</style></abstract><notes><style face="normal" font="default" size="100%">Accession Number: 4694993; Hornero, J. Martínez, I. Celestino, C. Gallego, F.J. Torres, V. Toribio, M.; Source Info: Jul2001, Vol. 162 Issue 4, p827; Subject Term: CORK oak; Subject Term: GENETIC polymorphisms; Number of Pages: 7p; Illustrations: 3 Charts, 3 Graphs; Document Type: Article; Full Text Word Count: 4881</style></notes><research-notes><style face="normal" font="default" size="100%">Accession Number: 4694993; Hornero, J. Martínez, I. Celestino, C. Gallego, F.J. Torres, V. Toribio, M.; Source Info: Jul2001, Vol. 162 Issue 4, p827; Subject Term: CORK oak; Subject Term: GENETIC polymorphisms; Number of Pages: 7p; Illustrations: 3 Charts, 3 Graphs; Document Type: Article; Full Text Word Count: 4881</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hornero, J.</style></author><author><style face="normal" font="default" size="100%">Martinez, I.</style></author><author><style face="normal" font="default" size="100%">Celestino, C.</style></author><author><style face="normal" font="default" size="100%">Gallego, F. J.</style></author><author><style face="normal" font="default" size="100%">Torres, V.</style></author><author><style face="normal" font="default" size="100%">Toribio, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">EARLY CHECKING OF GENETIC STABILITY OF CORK OAK SOMATIC EMBRYOS BY AFLP ANALYSIS.</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Plant Sciences</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">AFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA markers</style></keyword><keyword><style  face="normal" font="default" size="100%">in vitro culture</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">somaclonal variation</style></keyword><keyword><style  face="normal" font="default" size="100%">Somatic embryogenesis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2001///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://search.ebscohost.com/login.aspx?direct=true&amp;db=a9h&amp;AN=4694993&amp;lang=pt-br&amp;site=ehost-live&amp;scope=site</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">162</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Early checking of the genetic stability, of tissue culture-derived plants is necessary to obtain all the potential benefits of clonal forestry. Previous work in Quercus suber L. using random amplified polymorphic DNA (RAPD) markers showed no genetic variation among somatic embryos within embryogenic lines of zygotic origin. Genetic fingerprinting based on amplified fragment length polymorphisms (AFLP) allows the direct analysis of variation at the entire DNA level by generating more reproducible markers than RAPDs. To confirm the absence of genetic variation within six embryogenic lines of zygotic origin, six primer pairs were selected out of 48 combinations of primers for revealing up to 512 AFLP markers, 301 of them (58.8%) being polymorphic. The mean number of markers per genotype was 375. Again differences were recorded among embryogenic lines, even between those that arose from half-sib zygotic embryos, but no variation was observed among somatic embryos within embryogenic lines. To check variation in embryogenic lines raised from non-embryonic tissues, somatic embryogenesis was induced in expanding leaves collected from sprouts originating in three mature cork oak. DNA was extracted from leaves and from somatic embryos derived from each tree, and three primer pairs showed 165, 110, and 108 markers per genotype/tree, respectively. In one tree, AFLP patterns generated from leaves and somatic embryos were identical, but variation was detected in samples from the other two trees. Although the level of genetic variation detected in these lines (5.6% and 7.3% of polymorphism, respectively) is lower than that recorded for half sibs of cork oak (25%–31%), its influence on phenotypic variation needs further assessment. [ABSTRACT FROM AUTHOR]</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><notes><style face="normal" font="default" size="100%">Accession Number: 4694993; Hornero, J. Martínez, I. Celestino, C. Gallego, F.J. Torres, V. Toribio, M.; Source Info: Jul2001, Vol. 162 Issue 4, p827; Subject Term: CORK oak; Subject Term: GENETIC polymorphisms; Number of Pages: 7p; Illustrations: 3 Charts, 3 Graphs; Document Type: Article; Full Text Word Count: 4881Accession Number: 4694993; Hornero, J. Martínez, I. Celestino, C. Gallego, F.J. Torres, V. Toribio, M.; Source Info: Jul2001, Vol. 162 Issue 4, p827; Subject Term: CORK oak; Subject Term: GENETIC polymorphisms; Number of Pages: 7p; Illustrations: 3 Charts, 3 Graphs; Document Type: Article; Full Text Word Count: 4881The following values have no corresponding Zotero field:&lt;br/&gt;publisher: University of Chicago Press</style></notes></record></records></xml>