<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Pastorelli, Roberta</style></author><author><style face="normal" font="default" size="100%">Landi, Silvia</style></author><author><style face="normal" font="default" size="100%">Trabelsi, Darine</style></author><author><style face="normal" font="default" size="100%">Piccolo, Raimondo</style></author><author><style face="normal" font="default" size="100%">Mengoni, Alessio</style></author><author><style face="normal" font="default" size="100%">Bazzicalupo, Marco</style></author><author><style face="normal" font="default" size="100%">Pagliai, Marcello</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Effects of soil management on structure and activity of denitrifying bacterial communities</style></title><secondary-title><style face="normal" font="default" size="100%">Applied Soil Ecology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bacterial community</style></keyword><keyword><style  face="normal" font="default" size="100%">Denitriﬁcation</style></keyword><keyword><style  face="normal" font="default" size="100%">dgge</style></keyword><keyword><style  face="normal" font="default" size="100%">Managements</style></keyword><keyword><style  face="normal" font="default" size="100%">mRNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Sardinia</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier B.V.</style></publisher><volume><style face="normal" font="default" size="100%">49</style></volume><pages><style face="normal" font="default" size="100%">46-58</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The inﬂux of carbon sources and energy into the grossly oligotrophic soil system is a major driving force in biogeochemical cycles. Differences in these inputs due to the ground-covering plants and agriculture practices, likely inﬂuence the activity of the microbial communities. Previously, we have shown that the extent and degree of active bacterial diversity in soils are clearly affected by the type of management. In the present study we have evaluated the effects of different soil managements (tilled vineyard, grass-covered vineyard, temporary grassland, pasture, cork oak forest) on the diversity and activity of denitrifying bacterial species. Total DNA and RNA were directly extracted from soil sampled in two different seasons, spring and autumn. Genes and transcripts for two nitrite reductases (nirS and nirK), nitric oxide reductases (qnorB) and nitrous oxide reductase (nosZ) were used as PCR targets of denitrifying bacteria. Denaturing Gradient Gel Electrophoresis (DGGE) results showed a high abundance and wide distribution of bacterial denitriﬁers in soil,while denitrifying gene expressionwas detected in fewspecies or groups of species. A distinct seasonal shift was evident in denitrifying active communities, that also showed a signiﬁcant variation depending on soil variables such as texture, pH and organic carbon. DGGE band sequence analysis revealed similarities between sequences found in our soil and those belonging mainly to and -Protebacteria division. However, several sequences were not related to any known denitrifying species. The applied RT-nested PCR approach has proven to have the potential to provide information on diversity of active denitrifying bacterial communities and on where and when these bacteria express their ability within the soil.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Andreetta, Anna</style></author><author><style face="normal" font="default" size="100%">Macci, Cristina</style></author><author><style face="normal" font="default" size="100%">Ceccherini, Maria Teresa</style></author><author><style face="normal" font="default" size="100%">Cecchini, Guia</style></author><author><style face="normal" font="default" size="100%">Masciandaro, Graziana</style></author><author><style face="normal" font="default" size="100%">Pietramellara, Giacomo</style></author><author><style face="normal" font="default" size="100%">Carnicelli, Stefano</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Microbial dynamics in Mediterranean Moder humus</style></title><secondary-title><style face="normal" font="default" size="100%">Biology and Fertility of Soils</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">activities</style></keyword><keyword><style  face="normal" font="default" size="100%">dgge</style></keyword><keyword><style  face="normal" font="default" size="100%">enzyme</style></keyword><keyword><style  face="normal" font="default" size="100%">humus</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean forest soils</style></keyword><keyword><style  face="normal" font="default" size="100%">soil microbial communities</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.springerlink.com/index/10.1007/s00374-011-0622-9</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">48</style></volume><pages><style face="normal" font="default" size="100%">259 - 270</style></pages><isbn><style face="normal" font="default" size="100%">0037401106</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">There is a growing interest in the links between humus forms and soil biota, and little is known about these links in Mediterranean ecosystems. Culture-independent techniques, such as DNA extraction followed by DGGE and enzyme activities, allowed us to compare microbial communities in two horizons of a forest soil in different seasonal conditions. Direct in situ lysis was applied for extraction of DNA from soil; intracellular DNAwas separated from extracellular and used to represent the composition of microflora. The aims were to describe how biochemical and microbiological parameters correlate with topsoil properties in typical Mediterranean Moder humus. Changes in bacterial and fungal community composition were evident from DGGE profiles. Degrees of similarity and clustering correlation coefficients showed that the seasonal conditions may affect the composition and activity of bacterial and fungal communities in the OH horizon, while in the E horizon the two communities were hardly modified. In the same season, OH and E horizons showed a different composition of bacterial and fungal communities and different enzyme activities, suggesting similar behaviour of eubacteria and fungi relatively to all the variables analysed. Evidently, different organic carbon content in soil horizons influenced microflora composition and microbial activities involved in the P and N cycles.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Barrico, Lurdes</style></author><author><style face="normal" font="default" size="100%">Rodríguez-Echeverría, Susana</style></author><author><style face="normal" font="default" size="100%">Freitas, Helena</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Diversity of soil basidiomycete communities associated with Quercus suber L. in Portuguese montados</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Soil Biology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Basidiomycete</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehesa</style></keyword><keyword><style  face="normal" font="default" size="100%">dgge</style></keyword><keyword><style  face="normal" font="default" size="100%">diversity</style></keyword><keyword><style  face="normal" font="default" size="100%">montado</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2010///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S1164556310000294</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">46</style></volume><pages><style face="normal" font="default" size="100%">280 - 287</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The montado is an agro-silvo pastoral system characterized by an open oak formation combined with shrubs or a rotation of cultures/pastures/fallow. A key, but frequently overlooked, component of these systems is the fungal community associated with the dominant oaks. We present a study of the basidiomycete community in montado areas of Quercus suber L. from Central-Western Portugal, based on fruiting body assessment and PCR-DGGE of soil mycelium. Soil available phosphorus and organic matter content were also determined. The most frequent fruiting body found in the cork oak montados belonged to Lactarius, Clitocybe, Russula and Cortinarius species. Lactarius chrysorrheus was the most widely distributed species. Soil management practices cause a temporal increase in soil available phosphorus, and harrowing and fertilizer application also result in an increase in soil organic matter content. Mechanical clearing with recent soil disturbance had a negative effect on the richness and diversity of the basidiomycete community assessed using fruiting body. The molecular analysis of the basidiomycete community separated the sites with recent shrub-removal from the others sites. Our results show that soil management techniques that avoid disturbance of the top-soil layers are the best way to preserve the structure and diversity of the soil fungal community in the montado.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Elsevier Masson SAS</style></notes></record></records></xml>