<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Monson, Russell K.</style></author><author><style face="normal" font="default" size="100%">Jones, Ryan T.</style></author><author><style face="normal" font="default" size="100%">Rosenstiel, Todd N.</style></author><author><style face="normal" font="default" size="100%">Schnitzler, Jörg-Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Why only some plants emit isoprene.</style></title><secondary-title><style face="normal" font="default" size="100%">Plant, cell &amp; environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">ferns</style></keyword><keyword><style  face="normal" font="default" size="100%">phloem</style></keyword><keyword><style  face="normal" font="default" size="100%">phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">substrate</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">thermotolerance</style></keyword><keyword><style  face="normal" font="default" size="100%">ﬁtness</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2012///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22998549</style></url></web-urls></urls><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Isoprene (2-methyl-1,3-butadiene) is emitted from many plants and it appears to have an adaptive role in protecting leaves from abiotic stress. However, only some species emit isoprene. Isoprene emission has appeared and been lost many times independently during the evolution of plants. As an example, our phylogenetic analysis shows that isoprene emission is likely ancestral within the family Fabaceae (= Leguminosae), but that it has been lost at least 16 times and secondarily gained at least 10 times through independent evolutionary events. Within the division Pteridophyta (ferns), we conservatively estimate that isoprene emissions have been gained five times and lost two times through independent evolutionary events. Within the genus Quercus (oaks), isoprene emissions have been lost from one clade, but replaced by a novel type of light-dependent monoterpene emissions that uses the same metabolic pathways and substrates as isoprene emissions. This novel type of monoterpene emissions has appeared at least twice independently within Quercus, and has been lost from 9% of the individuals within a single population of Quercus suber. Gain and loss of gene function for isoprene synthase is possible through relatively few mutations. Thus, this trait appears frequently in lineages; but, once it appears, the time available for evolutionary radiation into environments that select for the trait is short relative to the time required for mutations capable of producing a non-functional isoprene synthase gene. The high frequency of gains and losses of the trait and its heterogeneous taxonomic distribution in plants may be explained by the relatively few mutations necessary to produce or lose the isoprene synthase gene combined with the assumption that isoprene emission is advantageous in a narrow range of environments and phenotypes.</style></abstract><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 22998549</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, J.</style></author><author><style face="normal" font="default" size="100%">Llusia, J.</style></author><author><style face="normal" font="default" size="100%">ASENSIO, D.</style></author><author><style face="normal" font="default" size="100%">MUNNÉ-BOSCH, S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Linking isoprene with plant thermotolerance, antioxidants and monoterpene emissions</style></title><secondary-title><style face="normal" font="default" size="100%">Plant, Cell &amp; Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antioxidants</style></keyword><keyword><style  face="normal" font="default" size="100%">ascorbic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">fumigation</style></keyword><keyword><style  face="normal" font="default" size="100%">Fv/Fm</style></keyword><keyword><style  face="normal" font="default" size="100%">high temperatures</style></keyword><keyword><style  face="normal" font="default" size="100%">isoprene</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthetic rates</style></keyword><keyword><style  face="normal" font="default" size="100%">thermotolerance</style></keyword><keyword><style  face="normal" font="default" size="100%">zeaxanthin</style></keyword><keyword><style  face="normal" font="default" size="100%">α-tocopherol</style></keyword><keyword><style  face="normal" font="default" size="100%">β-carotene</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.1365-3040.2004.01250.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">28</style></volume><pages><style face="normal" font="default" size="100%">278 - 286</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The purpose of the present study was to test the possible plant thermotolerance role of isoprene and to study its relationship with non-enzymatic antioxidants and terpene emissions. The gas exchange, chlorophyll fluorescence, extent of photo- and oxidative stress, leaf damage, mechanisms of photo- and antioxidant protection, and terpene emission were measured in leaves of Quercus ilex seedlings exposed to a ramp of temperatures of 5 °C steps from 25 to 50 °C growing with and without isoprene (10 µL L−1) fumigation. The results showed that isoprene actually conferred thermotolerance (shifted the decrease of net photosynthetic rates from 35 to 45 °C, increased Fv/Fm at 50 °C from 0.38 to 0.65, and decreased the leaf area damaged from 27 to 15%), that it precluded or delayed the enhancement of the antioxidant non-enzymatic defence conferred by α-tocopherol, ascorbic acid or β-carotene consumption in response to increasing temperatures, and that it decreased by approximately 70% the emissions of monoterpenes at the highest temperatures. This suggests that there are inducible mechanisms triggered by the initial stages of thermal damage that up-regulate these antioxidant compounds at high temperatures and that these mechanisms are somehow suppressed in the presence of exogenous isoprene, which seems to already exert an antioxidant-like behaviour.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Llusia, Joan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Linking photorespiration, monoterpenes and thermotolerance in Quercus</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">electron transport rate (ETR)</style></keyword><keyword><style  face="normal" font="default" size="100%">fumigation</style></keyword><keyword><style  face="normal" font="default" size="100%">Fv : Fm</style></keyword><keyword><style  face="normal" font="default" size="100%">limonene</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">photochemical reflectance index (PRI)</style></keyword><keyword><style  face="normal" font="default" size="100%">photorespiration</style></keyword><keyword><style  face="normal" font="default" size="100%">thermotolerance</style></keyword><keyword><style  face="normal" font="default" size="100%">α-pinene</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2002///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1046/j.1469-8137.2002.00457.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">155</style></volume><pages><style face="normal" font="default" size="100%">227 - 237</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • The functions of two important plant processes, photorespiration and monoterpene production remain controversial. Here, we investigated one possible function, that of protection of plants from photodamage at high temperatures. * • Fluorescence, reflectance, monoterpene concentrations and visual leaf damage were measured in Quercus ilex seedlings exposed to temperature increases from 25 to 50°C (in 5°C steps) under photorespiratory (21% O 2 ) or nonphotorespiratory (2% O 2 ) atmospheres, and under control or terpene fumigation conditions. * • Lower variable to maximum fluorescence ratio (Fv : Fm: potential photochemical efficiency of photosystem II, PSII) and electron transport rate (ETR) were found in nonphotorespiratory conditions at temperatures greater than 35°C. Monoterpene concentrations were also lower, and leaf damage greater, in the low O 2 atmospheres. Monoterpene fumigation, which increased the foliar terpene concentrations by two- to four-fold, increased the photochemical efficiency between 35°C and 50°C, and decreased leaf damage, only under the nonphotorespiratory conditions. * • These results provide evidence that: photorespiration decreases photodamage, especially at high temperatures; photorespiration increases monoterpene production; plants are able to acquire exogenous monoterpenes and the acquisition response to temperature follows the stomatal conductance response; and monoterpenes can replace photorespiration in protection from photodamage at high temperatures, possibly by scavenging oxygen-reactive species, but they do not provide additional thermotolerance.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Delfine, Sebastiano</style></author><author><style face="normal" font="default" size="100%">Csiky, Olav</style></author><author><style face="normal" font="default" size="100%">Seufert, Guenther</style></author><author><style face="normal" font="default" size="100%">Loreto, Francesco</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Fumigation with exogenous monoterpenes of a non-isoprenoid-emitting oak (Quercus suber): monoterpene acquisition, translocation, and effect on the photosynthetic properties at high temperatures</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">isoprenoid emission</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">thermotolerance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.wiley.com/10.1046/j.1469-8137.2000.00612.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">146</style></volume><pages><style face="normal" font="default" size="100%">27 - 36</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We tested if fumigation with exogenous monoterpenes might induce thermotolerance in leaves of an oak species (Quercus suber) which does not form and emit isoprenoids. To understand if exogenous monoterpene fumigation results in internal accumulation of monoterpenes, a physical method of monoterpene extraction was used. The internal content of monoterpenes increased in concert with increasing fumigation doses. This unambiguously demonstrated acquisition of exogenous monoterpenes. We exposed fumigated Q. suber leaves to two cycles of increasing temperatures from 35 to 55°C at 5°C steps. When leaves were exposed to a low dose of exogenous monoterpenes, yielding an internal content similar to that endogenously formed in the leaves of the monoterpene- emitter Q. ilex, no clear improvement in thermotolerance was found. When leaves were exposed to a high dose of exogenous monoterpenes, yielding an internal content of about ®ve fold the endogenous pool of Q. ilex, but comparable with the expected content following stress-induced stomatal closure, photosynthesis inhibition at high temperatures was attenuated. This eﬀect was observed only at temperatures !45°C during the ®rst cycle, but at all temperatures between 35 and 55°C when plants were exposed to two cycles of high temperatures. Monoterpenes were still found in the leaves of Q. suber 12 h after ending the fumigation. Monoterpenes were also found in non-fumigated leaves distant up to 45 cm from the fumigated leaves. If monoterpenes make the photosynthetic apparatus more resistant to high temperatures, the eﬀect might not be limited to the fumigated leaves and might be persistent after fumigation.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Loreto, F.</style></author><author><style face="normal" font="default" size="100%">Förster, A.</style></author><author><style face="normal" font="default" size="100%">Dürr, M.</style></author><author><style face="normal" font="default" size="100%">Csiky, O.</style></author><author><style face="normal" font="default" size="100%">Seufert, G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">On the monoterpene emission under heat stress and on the increased thermotolerance of leaves of Quercus ilex L. fumigated with selected monoterpenes</style></title><secondary-title><style face="normal" font="default" size="100%">Plant, Cell &amp; Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">monoterpene emission</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene fumigation</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">respiration</style></keyword><keyword><style  face="normal" font="default" size="100%">thermotolerance</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1998///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1046/j.1365-3040.1998.00268.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">21</style></volume><pages><style face="normal" font="default" size="100%">101 - 107</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Leaves of the monoterpene emitter Quercus ilex were exposed to a temperature ramp with 5 °C steps from 30 to 55 °C while maintained under conditions in which endogenous emission of monoterpenes was allowed or suppressed, or under fumigation with selected exogenous monoterpenes. Fumigation with monoterpenes reduced the decline of photosynthesis, photorespiration and monoterpene emission found in non-fumigated leaves exposed to high temperatures. It also substantially increased respiration when photosynthesis and photorespiration were inhibited by low O2 and CO2-free air. These results indicate that, as previously reported for isoprene, monoterpenes may help plants cope with heat stress. Monoterpenes may enhance membrane stability, thus providing a rather non-specific protection of photosynthetic and respiratory processes. Monoterpene emission was maximal at a temperature of 35 °C and was inhibited at higher temperatures. This is likely to be the result of the temperature dependency of the enzymes involved in monoterpene synthesis. In contrast to other monoterpenes, cis- and trans-β-ocimene did not respond to exposure to high temperatures. Cis-β-ocimene also did not respond to low O2 or to fumigation. These results indicate that cis and trans-β-ocimene may have a different pathway of formation that probably does not involve enzymatic synthesis.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Publishing Ltd</style></notes></record></records></xml>