<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Correia, Barbara</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Meijón, Mónica</style></author><author><style face="normal" font="default" size="100%">Rodriguez, José Luis</style></author><author><style face="normal" font="default" size="100%">Dias, Maria Celeste</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Cañal, Maria Jesus</style></author><author><style face="normal" font="default" size="100%">Rodriguez, Roberto</style></author><author><style face="normal" font="default" size="100%">Pinto, Glória</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS ONE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acclimatization</style></keyword><keyword><style  face="normal" font="default" size="100%">Acclimatization: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Blotting</style></keyword><keyword><style  face="normal" font="default" size="100%">CHROMATIN</style></keyword><keyword><style  face="normal" font="default" size="100%">CORK oak (citation</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine: analogs &amp; derivatives</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA methylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrolytes</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrolytes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Epigenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat-Shock Response</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat-Shock Response: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Histones</style></keyword><keyword><style  face="normal" font="default" size="100%">Histones: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">POST-translational modification</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Random Amplified Polymorphic DNA Technique</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">tags)</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">TREES -- Research</style></keyword><keyword><style  face="normal" font="default" size="100%">Western</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3543447&amp;tool=pmcentrez&amp;rendertype=abstracthttp://dx.doi.org/10.1371/journal.pone.0053543</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">8</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Trees necessarily experience changes in temperature, requiring efficient short-term strategies that become crucial in environmental change adaptability. DNA methylation and histone posttranslational modifications have been shown to play a key role in both epigenetic control and plant functional status under stress by controlling the functional state of chromatin and gene expression. Cork oak (Quercus suber L.) is a key stone of the Mediterranean region, growing at temperatures of 45°C. This species was subjected to a cumulative temperature increase from 25°C to 55°C under laboratory conditions in order to test the hypothesis that epigenetic code is related to heat stress tolerance. Electrolyte leakage increased after 35°C, but all plants survived to 55°C. DNA methylation and acetylated histone H3 (AcH3) levels were monitored by HPCE (high performance capillary electrophoresis), MS-RAPD (methylation-sensitive random-amplified polymorphic DNA) and Protein Gel Blot analysis and the spatial distribution of the modifications was assessed using a confocal microscope. DNA methylation analysed by HPCE revealed an increase at 55°C, while MS-RAPD results pointed to dynamic methylation-demethylation patterns over stress. Protein Gel Blot showed the abundance index of AcH3 decreasing from 25°C to 45°C. The immunohistochemical detection of 5-mC (5-methyl-2′-deoxycytidine) and AcH3 came upon the previous results. These results indicate that epigenetic mechanisms such as DNA methylation and histone H3 acetylation have opposite and particular dynamics that can be crucial for the stepwise establishment of this species into such high stress (55°C), allowing its acclimation and survival. This is the first report that assesses epigenetic regulation in order to investigate heat tolerance in forest trees.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Public Library of Science&lt;br/&gt;accession-num: 23326451</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Espelta, Josep Maria</style></author><author><style face="normal" font="default" size="100%">Cortes, P.</style></author><author><style face="normal" font="default" size="100%">Molowny-Horas, R.</style></author><author><style face="normal" font="default" size="100%">Retana, J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Acorn crop size and pre-dispersal predation determine inter-specific differences in the recruitment of co-occurring oaks.</style></title><secondary-title><style face="normal" font="default" size="100%">Oecologia</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Feeding Behavior</style></keyword><keyword><style  face="normal" font="default" size="100%">Feeding Behavior: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">Germination: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Models, Biological</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Species Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">weevils</style></keyword><keyword><style  face="normal" font="default" size="100%">Weevils: physiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19544074</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">161</style></volume><pages><style face="normal" font="default" size="100%">559 - 68</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The contribution of pre-dispersal seed predation to inter-specific differences in recruitment remains elusive. In species with no resistance mechanisms, differences in pre-dispersal predation may arise from differences in seed abundance (plant satiation) or in the ability of seeds to survive insect infestation (seed satiation). This study aimed to analyse the impact of pre-dispersal acorn predation by weevils in two co-occurring Mediterranean oaks (Quercus ilex and Quercus humilis) and to compare its relevance with other processes involved in recruitment. We monitored the patterns of acorn production and acorn infestation by weevils and we conducted experimental tests of acorn germination after weevil infestation, post-dispersal predation and seedling establishment in mixed forests. Monitoring and experimental data were integrated in a simulation model to test for the effects of pre-dispersal predation in recruitment. In both oaks pre-dispersal acorn infestation decreased with increasing acorn crop size (plant satiation). This benefited Q. ilex which exhibited stronger masting behaviour than Q. humilis, with almost a single and outstanding reproductive event in 6 years. Acorn infestation was more than twice as high in Q. humilis (47.0%) as in Q. ilex (20.0%) irrespective of the number of seeds produced by each species. Although germination of infested acorns (seed satiation) was higher in Q. humilis (60%) than in Q. ilex (21%), this could barely mitigate the higher infestation rate in the former species, to reduce seed loss. Conversely to pre-dispersal predation, no inter-specific differences were observed either in post-dispersal predation or seedling establishment. Our results indicate that pre-dispersal predation may contribute to differences in seed supply, and ultimately in recruitment, between co-existing oaks. Moreover, they suggest that seed satiation can barely offset differences in seed infestation rates. This serves as a warning against overemphasising seed satiation as a mechanism to overcome seed predation by insects.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 19544074</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarez, Rubén</style></author><author><style face="normal" font="default" size="100%">Alvarez, José M.</style></author><author><style face="normal" font="default" size="100%">Humara, Jaime M.</style></author><author><style face="normal" font="default" size="100%">Revilla, Angeles</style></author><author><style face="normal" font="default" size="100%">Ordás, Ricardo J.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.</style></title><secondary-title><style face="normal" font="default" size="100%">Biotechnology letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acetyltransferases</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetyltransferases: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Dosage</style></keyword><keyword><style  face="normal" font="default" size="100%">Genomic Instability</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicide Resistance</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides</style></keyword><keyword><style  face="normal" font="default" size="100%">Herbicides: toxicity</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants, Genetically Modified: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plasmids</style></keyword><keyword><style  face="normal" font="default" size="100%">Promoter Regions, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium</style></keyword><keyword><style  face="normal" font="default" size="100%">Rhizobium: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Transformation, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays</style></keyword><keyword><style  face="normal" font="default" size="100%">Zea mays: genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19543858</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">31</style></volume><pages><style face="normal" font="default" size="100%">1477 - 83</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.</style></abstract><issue><style face="normal" font="default" size="100%">9</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 19543858</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Castro-Díez, Pilar</style></author><author><style face="normal" font="default" size="100%">Navarro, Javier</style></author><author><style face="normal" font="default" size="100%">Pintado, Ana</style></author><author><style face="normal" font="default" size="100%">Sancho, Leopoldo G.</style></author><author><style face="normal" font="default" size="100%">Maestro, Melchor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Interactive effects of shade and irrigation on the performance of seedlings of three Mediterranean Quercus species</style></title><secondary-title><style face="normal" font="default" size="100%">Tree Physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agriculture</style></keyword><keyword><style  face="normal" font="default" size="100%">Agriculture: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass allocation</style></keyword><keyword><style  face="normal" font="default" size="100%">carbon assimilation rate</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenology</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Relative growth rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: pharmacology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16356909http://treephys.oxfordjournals.org/content/26/3/389.abstract</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">26</style></volume><pages><style face="normal" font="default" size="100%">389 - 400</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Shade and irrigation are frequently used to increase the success of Mediterranean Quercus spp. plantations. However, there is controversy about the combined effects of these treatments on plant performance. We assessed the effects of two irradiances (full sunlight and moderate shade) and two summer watering regimes (high (daily) and low (alternate days)) on leaf and whole-plant traits of 1-year-old seedlings of Quercus coccifera, Q. ilex subsp. ballota and Q. faginea grown outdoors for 8.5 months. Leaf traits included measures of morphology, nitrogen concentration, gas exchange and photochemical efficiency, and measures of whole-plant traits included biomass allocation patterns, growth phenology, across-summer leaf area change and relative growth rate (RGR).Moderate shade reduced leaf mass per area, increased photochemical efficiency, maximum carbon assimilation rate (Amax) and allocation to leaves, and prolonged the growing period in one or more of the species. Daily watering in summer increased Amax of Q. ilex and prolonged the growing period of Q. ilex and Q. faginea. Both treatments tended to increase RGR. The effect of shade was greater in the low-watering regime than in the high-watering regime for two of the 15 studied traits, with treatment effects being independent for the remaining 13 traits. Leaf nitrogen and the ability to maintain leaf area after the arid period, rather than biomass allocation traits, explained the variation in seedling RGR. Trait responsiveness to the treatments was low and similar among species and between study scales, being unexpectedly low in Q. faginea leaves. This may be because selective pressures on leaf plasticity act differently in deciduous and evergreen species. We conclude that moderate shade and daily summer watering enhance the performance of Mediterranean Quercus seedlings through species-specific mechanisms.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Interactive effects of shade and irrigation on the performance of seedlings of three Mediterranean Quercus species - Castro-Díez, Pilar; Navarro, Javier; Pintado, Ana; Sancho, Leopoldo G; Maestro, Melchor)From Duplicate 2 (Interactive effects of shade and irrigation on the performance of seedlings of three Mediterranean Quercus species - Castro-Díez, Pilar; Navarro, Javier; Pintado, Ana; Sancho, Leopoldo G; Maestro, Melchor)The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 16356909</style></notes></record></records></xml>