<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valero-Galvàn, José</style></author><author><style face="normal" font="default" size="100%">González-Fernández, Raquel</style></author><author><style face="normal" font="default" size="100%">Navarro-Cerrillo, Rafael Maria</style></author><author><style face="normal" font="default" size="100%">Gil-Pelegrín, Eustaquio</style></author><author><style face="normal" font="default" size="100%">Jorrín-Novo, Jesús V</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of Proteome Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">Chlorophyll ﬂuorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">drought stress in Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Droughts</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gel</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Expression Regulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Proteins: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics</style></keyword><keyword><style  face="normal" font="default" size="100%">Proteomics: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Tandem mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Two-Dimensional</style></keyword><keyword><style  face="normal" font="default" size="100%">water potential</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year></dates><publisher><style face="normal" font="default" size="100%">American Chemical Society</style></publisher><volume><style face="normal" font="default" size="100%">12</style></volume><pages><style face="normal" font="default" size="100%">5110-5123</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Responses to drought stress by water withholding have been studied in 1 year old Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) seedlings from seven provenances from Andalusia (southern Spain). Several physiological parameters, including predawn xylem water potentials and relative water content in soil, roots, and leaves as well as maximum quantum efficiency and yield of PSII were evaluated for 28 days in both irrigated and nonirrigated seedlings. The leaf proteome map of the two provenances that show the extreme responses (Seville, GSE, is the most susceptible, while Almer??a, SSA, is the least susceptible) was obtained. Statistically significant variable spots among provenances and treatments were subjected to MALDI-TOF/TOF-MS/MS analysis for protein identification. In response to drought stress, ?12.4% of the reproducible spots varied significantly depending on the treatment and the population. These variable proteins were mainly chloroplastic and belonged to the metabolism and defense/stress functional categories. The 2-DE protein profile of nonirrigated seedlings was similar in both provenances. Physiological and proteomics data were generally in good agreement. The general trend was a decrease in protein abundance upon water withholding in both provenances, mainly in those involved in ATP synthesis and photosynthesis. This decrease, moreover, was most marked in the most susceptible population compared with the less susceptible one.</style></abstract><accession-num><style face="normal" font="default" size="100%">24088139</style></accession-num><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></notes><research-notes><style face="normal" font="default" size="100%">From Duplicate 2 (Physiological and Proteomic Analyses of Drought Stress Response in Holm Oak Provenances - Valero-Galván, José; González-Fernández, Raquel; Navarro-Cerrillo, Rafael Maria; Gil-Pelegrín, Eustaquio; Jorrín-Novo, Jesús V)</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Ogaya, Romà</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Asensio, Dolores</style></author><author><style face="normal" font="default" size="100%">Llusia, Joan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Chlorophyll fluorescence responses to temperature and water availability in two co-dominant Mediterranean shrub and tree species in a long-term field experiment simulating climate change</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental and Experimental Botany</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chlorophyll ﬂuorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">climate change</style></keyword><keyword><style  face="normal" font="default" size="100%">Cold</style></keyword><keyword><style  face="normal" font="default" size="100%">Drought</style></keyword><keyword><style  face="normal" font="default" size="100%">evergreen leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Fv/Fm</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean forest</style></keyword><keyword><style  face="normal" font="default" size="100%">phillyrea latifolia</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S009884721100178X</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">73</style></volume><pages><style face="normal" font="default" size="100%">89 - 93</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A rain exclusion experiment simulating drought conditions expected in Mediterranean areas for the following decades (15% decrease in soil moisture) is being conducted since 1999 in a Mediterranean holm oak forest to study its response to the forecasted climatic changes for the coming decades. The maximum PSII quantum yield of primary photochemistry (Fv/Fm) was measured in Quercus ilex, and Phillyrea latifolia, the co-dominant species of the studied forest, from 1999 to 2009 in four plots: two of them were control plots and the other two plots received the rain exclusion treatment. In both species, the Fv/Fm values were highly dependent on air temperatures, and in a second term, in water availability. P. latifolia was the species with the larger decrease in Fv/Fm values induced by low air temperatures, while in hot seasons, the Fv/Fm values in P. latifolia were even higher than in Q. ilex. Rainfall exclusion decrease Fv/Fm values signiﬁcantly only in few monitoring dates. The most drought resistant species P. latifolia was more affected by the experimental rainfall exclusion than Q. ilex that instead lost number of leaves per tree. There was a synergic effect of drought stress and winter cold in P. latifolia not observed in Q. ilex, but a more conservative strategy in P. latifolia maintaining leaves with a down-regulation of the linear photosynthetic electron transport. These results indicate that, although other physiological and reproductive strategies at whole plant level must be also taken into account, the warmer and drier environment expected for the following decades could favour the species more sensitive to cold and more resistant to drought, the shrub P. latifolia, in detriment of the tree Q. ilex as already observed in the ﬁeld after severe heat-drought episodes.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Oliveira, G</style></author><author><style face="normal" font="default" size="100%">Penuelas, J</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Comparative photochemical and phenomorphological responses to winter stress of an evergreen ( Quercus ilex L.) and a semi-deciduous ( Cistus albidus</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Oecologica</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Chlorophyll ﬂuorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">evergreens</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf characteristics</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean winter</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenology</style></keyword><keyword><style  face="normal" font="default" size="100%">photoinhibition</style></keyword><keyword><style  face="normal" font="default" size="100%">semi-deciduous</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2000</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2000///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S1146609X00001211</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">21</style></volume><pages><style face="normal" font="default" size="100%">97 - 107</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The impact of winter stress on plants from a Mediterranean area was evaluated through comparison of photosystem II (PS II) efficiencies and phenomorphological characteristics of two Mediterranean woody species – Quercus ilex (evergreen) and Cistus albidus (semi-deciduous). The studies were carried out in NE Spain, at two sites with different mean winter temperatures. The results showed that reductions of the efficiency of PS II may occur in Mediterranean plant communities during winter, and are especially remarkable on colder days. The extent and duration of the decrease in PS II efficiency (photoinhibition) depended not only on the climatic conditions, but also on the site, on the species considered and on the position of leaves in the plant canopy. Increased photoinhibition at the colder site was only clear for C. albidus. Nevertheless, the efficiencies of PS II were always higher in C. albidus than in Q. ilex. Phenological patterns, morphology and leaf inclination may protect C. albidus leaves from potentially photoinhibitory conditions in winter. Morphological and structural photoprotection is apparently not so well developed in Q. ilex, which probably relies more on increased protection at the photochemical level of its long-lived leaves. As has been reported in relation to summer-drought stress, Q. ilex has possibly developed a strategy of tolerance to photoinhibition, whereas C. albidus relies preferentially on avoidance features</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue></record></records></xml>