<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarado, P.</style></author><author><style face="normal" font="default" size="100%">Manjon, J. L.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A quantitative and molecular examination of Tuber melanosporum mycorrhizae in Quercus ilex seedlings from different suppliers in Spain</style></title><secondary-title><style face="normal" font="default" size="100%">FOREST SYSTEMS</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Certification</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">mycorrhiza</style></keyword><keyword><style  face="normal" font="default" size="100%">TRFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">truffle</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">22</style></volume><pages><style face="normal" font="default" size="100%">159 - 169</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Aim of study: The aim of the work was to determine the degree of mycorrhization of Quercus ilex L. subsp. ballota (Desf.) Samp. by the black truffle fungus T. melanosporum Vittad. by quantitative and molecular analyses. Area of study: seedlings inoculated by different Spanish suppliers. Material and methods: The internal transcribed spacers (ITS) of mycorrhizae from different plants were amplified by nested PCR involving fluorescently-labelled primers, and the amplicons either directly sized by ARISA or analysed by TRFLP following their digestion with restriction endonucleases. TRFLP analysis distinguished between mycorrhizae of T melanosporum, T. indicum Cooke &amp; Massee and T. borchii Vittad., as suggested possible by virtual (in silico) TRFLP analysis and real TRFLP analysis of the ascomata of these species. Main results: Significant differences between suppliers were detected in terms of the mean number of mycorrhizae established per plant and percentage mycorrhization. These results allowed the following quality standards for 2 year-old plants to be proposed: a) good quality: &gt;3,000 mycorrhizae/plant, &gt;40% mycorrhization, b) medium (acceptable): &gt;3,000 mycorrhizae/plant, &gt;30% mycorrhization, c) low quality: &lt;3,000 mycorrhizae/plant or &lt;30% mycorrhization, always supposing the mycorrhizae counted represent the species of interest as confirmed by the presence of its DNA and the absence of DNA belonging to contaminating species. Finally, a new microsatellite allelic map obtained from the analysis of several T melanosporum populations across Spain was used to provide a tool capable of determining the geographic origin of the fungi used to inoculate plants. Research highlights: The proposed quality standards can be useful for the evaluation and certification of commercialized Q. ilex plants mycorrhized with T. melanosporum.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: CTRA CORUNA KM 7 5, MADRID, 28040, SPAIN&lt;br/&gt;publisher: INST NACIONAL INVESTIGACION TECHNOLOGIA AGRARIA ALIMENTARIA</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Garcia-Barreda, S.</style></author><author><style face="normal" font="default" size="100%">Reyna, S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Short-term dynamics of Quercus ilex advance regeneration in a Pinus nigra plantation after the creation of small canopy gaps</style></title><secondary-title><style face="normal" font="default" size="100%">FOREST SYSTEMS</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">group selection cutting</style></keyword><keyword><style  face="normal" font="default" size="100%">mediterranean forest</style></keyword><keyword><style  face="normal" font="default" size="100%">seedling resprout</style></keyword><keyword><style  face="normal" font="default" size="100%">stand initiation</style></keyword><keyword><style  face="normal" font="default" size="100%">truffle</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">22</style></volume><pages><style face="normal" font="default" size="100%">179 - 188</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Aim of the study: The aim of the research is to analyse the role of Quercus ilex advance regeneration in the stand regeneration of pine plantations after small-sized canopy openings, and to assess the influence of the forest stand and the canopy opening. The performance of the advance regeneration under the pine plantation is also examined. Area of study: A Pinus nigra plantation in dry Continental Mediterranean climate in eastern Spain. Material and methods: The tree regeneration of ten canopy openings of 0.17-0.43 ha was monitored during five years after treatment. It was also sampled in 0.12 ha-plots in the non-treated pine plantation surrounding the openings. Main results: An important increase in the height of Q. ilex regeneration was observed in the openings, unlike what was found in the intact pine plantation. In the pine plantation, stand density showed a moderate positive influence on the density of Q. ilex regeneration, whereas in the canopy gaps Q. ilex height was negatively influenced by stand density before the opening. Research highlights: The canopy opening triggered a response in Q. ilex advance regeneration, although height growth rates seemed to reduce over time. The results support the view that promoting Q. ilex in pine plantations may require different management strategies depending on the characteristics of the pine overstorey and on the density and size of the advance regeneration.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: CTRA CORUNA KM 7 5, MADRID, 28040, SPAIN&lt;br/&gt;publisher: INST NACIONAL INVESTIGACION TECHNOLOGIA AGRARIA ALIMENTARIA</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Suz, Laura M.</style></author><author><style face="normal" font="default" size="100%">Martín, María P.</style></author><author><style face="normal" font="default" size="100%">Oliach, Daniel</style></author><author><style face="normal" font="default" size="100%">Fischer, Christine R.</style></author><author><style face="normal" font="default" size="100%">Colinas, Carlos</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mycelial abundance and other factors related to truffle productivity in Tuber melanosporum–Quercus ilex orchards</style></title><secondary-title><style face="normal" font="default" size="100%">FEMS Microbiology Letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">burn</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA soil mycelium</style></keyword><keyword><style  face="normal" font="default" size="100%">real-time PCR</style></keyword><keyword><style  face="normal" font="default" size="100%">rock cover</style></keyword><keyword><style  face="normal" font="default" size="100%">truffle</style></keyword><keyword><style  face="normal" font="default" size="100%">Tuber melanosporum</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.1574-6968.2008.01213.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">285</style></volume><pages><style face="normal" font="default" size="100%">72 - 78</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Relative quantification of DNA from Tuber melanosporum mycelia was performed by conventional and real-time PCR in soil from trees in three truffle orchards of different ages to determine: (1) whether burn appearance is related to the amount of T. melanosporum mycelium in soil, and (2) whether productivity onset and truffle production are related to (a) the amount of T. melanosporum mycelium in soil, (b) tree height and diameter, (c) burn extension and (d) surface rock cover. The burn seems to appear only after a certain amount of mycelium has formed. Precociously productive trees presented higher quantities of mycelium than nonproductive trees in the productivity onset study, while highly productive trees presented less quantities of mycelium than nonproductive trees in the productivity study. Trees with high but not excessive surface rock cover showed greater truffle production. Larger trees tended to display a burn earlier than smaller trees.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Publishing Ltd</style></notes></record></records></xml>