<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Papa, S</style></author><author><style face="normal" font="default" size="100%">Bartoli, G</style></author><author><style face="normal" font="default" size="100%">Nacca, F</style></author><author><style face="normal" font="default" size="100%">D'Abrosca, B</style></author><author><style face="normal" font="default" size="100%">Cembrola, E</style></author><author><style face="normal" font="default" size="100%">Pellegrino, a</style></author><author><style face="normal" font="default" size="100%">Fiorentino, a</style></author><author><style face="normal" font="default" size="100%">Fuggi, A</style></author><author><style face="normal" font="default" size="100%">Fioretto, a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Trace metals, peroxidase activity, PAHs contents and ecophysiological changes in Quercus ilex leaves in the urban area of Caserta (Italy).</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of environmental management</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Amino acid contents</style></keyword><keyword><style  face="normal" font="default" size="100%">PAHs</style></keyword><keyword><style  face="normal" font="default" size="100%">peroxidase</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Trace Elements</style></keyword><keyword><style  face="normal" font="default" size="100%">Urban area</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><publisher><style face="normal" font="default" size="100%">Elsevier Ltd</style></publisher><volume><style face="normal" font="default" size="100%">113</style></volume><pages><style face="normal" font="default" size="100%">501-509</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Trace metals and polycyclic aromatic hydrocarbons, severely affecting human, animal and plants health, highly contribute to the air pollution in urban areas mainly due to car traffic. In this study the air biomonitoring of the city of Caserta (South Italy) has been performed by using Quercus ilex L., a widespread ornamental plant in parks, gardens and avenues. The plant leaves from different sites within the urban area were collected and used to determine the concentrations of V, Cd, Cr, Pb, Ni, Cu, and PAHs as well as the free amino acid content and peroxidase enzyme activity as indices of the leaf physiological conditions. All the tested trace metals showed concentrations higher than the control site. Lead was positively correlated to Cd and Cr and showed, also, a positive trend with Ni and Cu that, in their turn, were highly correlated between them. Positive and significant correlations were evidenced between total PAHs and carcinogenic PAHs and negative correlations between those and all trace metals assayed except V. Cu and Cd contents evidence negative correlations with peroxidase activity, and the free amino acid contents. The PAHs, in particular Carc-PAHs, were negatively correlated to the tested heavy metals. POD was positively correlated only with V and negatively correlated with Cu and Cd.</style></abstract><accession-num><style face="normal" font="default" size="100%">22868269</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Echevarría-Zomeño, Sira</style></author><author><style face="normal" font="default" size="100%">Abril, Nieves</style></author><author><style face="normal" font="default" size="100%">Ruiz-Laguna, Julia</style></author><author><style face="normal" font="default" size="100%">Jorrín-novo, Jesús</style></author><author><style face="normal" font="default" size="100%">Maldonado-Alconada, Ana M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Simple, rapid and reliable methods to obtain high quality RNA and genomic DNA from Quercus ilex L. leaves suitable for molecular biology studies</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Physiologiae Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">á high-quality rna</style></keyword><keyword><style  face="normal" font="default" size="100%">dna and rna</style></keyword><keyword><style  face="normal" font="default" size="100%">dna and rna extraction</style></keyword><keyword><style  face="normal" font="default" size="100%">extraction á genomic dna</style></keyword><keyword><style  face="normal" font="default" size="100%">genomic dna</style></keyword><keyword><style  face="normal" font="default" size="100%">high-quality rna</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">quercus ilex leaves á</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2011</style></year></dates><volume><style face="normal" font="default" size="100%">34</style></volume><pages><style face="normal" font="default" size="100%">793-805</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Isolation of high-quality RNA and genomic DNA (gDNA) from many samples is a necessary step before accomplishing molecular biology studies. The particular composition of Quercus ilex leaves, specially hard and rich in cell wall material, polyphenolics and secondary metabolites, usually results in preparations contaminated with non-nucleic acid compounds. Although many methods have been developed, each case of study demands a protocol adapted to the speciﬁc plant sample and the pursued research objectives. We have evaluated several protocols to establish the methodology that best suited to our current genetic and molecular studies on Q. ilex. Our priority was to select the simplest methods reducing the plant starting material and the time employed, without compromising yield, quality and integrity of the isolated nucleic acids. Our results point to two protocols based on silicamembrane puriﬁcation, as the most convenient for Q. ilex leaf tissue, and both procedures are greatly improved by adding insoluble polyvinyl polypyrrolidone during the isolation process. The protocols optimized here can be completed at the microfuge scale and allow a researcher to process 48 samples in 1 h, producing high quality preparations suitable for the routinely molecular biology applications with higher efﬁciency than other more labour and time-consuming protocols</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Monaci, F</style></author><author><style face="normal" font="default" size="100%">Bargagli, R</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Barium and other trace metals as indicators of vehicle emissions</style></title><secondary-title><style face="normal" font="default" size="100%">WATER AIR AND SOIL POLLUTION</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">accumulative bioindication</style></keyword><keyword><style  face="normal" font="default" size="100%">Central Italy</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Siena</style></keyword><keyword><style  face="normal" font="default" size="100%">trace element pollution</style></keyword><keyword><style  face="normal" font="default" size="100%">Urban environment</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1997</style></year></dates><publisher><style face="normal" font="default" size="100%">KLUWER ACADEMIC PUBL</style></publisher><pub-location><style face="normal" font="default" size="100%">SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS</style></pub-location><volume><style face="normal" font="default" size="100%">100</style></volume><pages><style face="normal" font="default" size="100%">89-98</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In the Siena urban environment, unwashed Q when leaves had a significantly higher Al, Ba, Cr, Fe, Mn, Na, Ni, Pb, Ti, and V content than samples from a neighbouring Mediterranean maquis. Significant relationships between element pairs indicated both the presence of metals emitted by motor vehicles and lithophilic elements, presumably from soil particles, resuspended by wind or turbulence generated by traffic. In order to minimize the effects of soil dust contamination and to seek clues to the metal sources, the estimated substrate contribution was subtracted from the raw concentrations of elements in Q. ilex leaves. Normalization for local soil composition confirmed that automotive emissions are the principal source of pollution in Siena. On the basis of a very significant relationship between normalized concentrations of Pb and Ba and the presence of the latter element in diesel and unleaded gasoline, Ba was recognized as a valuable tracer for vehicle emissions, in the place of Pb.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valerio, F</style></author><author><style face="normal" font="default" size="100%">Brescianini, C</style></author><author><style face="normal" font="default" size="100%">Lastraioli, S</style></author><author><style face="normal" font="default" size="100%">Coccia, S</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metals in Leaves as Indicators of Atmospheric Pollution in Urban Areas</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Environmental Analytical Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">atmospheric pollution</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy metals (PG)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex leaves</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1989</style></year></dates><publisher><style face="normal" font="default" size="100%">Taylor &amp; Francis</style></publisher><volume><style face="normal" font="default" size="100%">37</style></volume><pages><style face="normal" font="default" size="100%">245-251</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract The use of biological indicators to monitor atmospheric pollution is widely distributed. The analysis of heavy metals in leaves is suggested as a method for a rapid identification of polluted areas. Reproducibility and representativity of this method must be assessed. We have verified if the concentration of lead, chromium, copper, nickel and manganese in ?Quercus Ilex? leaves may be used to classify urban areas according to the level of their atmospheric pollution. Particular emphasis was placed on reproducibility of sampling and the analytical method. Preliminary results show a linear correlation between concentration of metals in atmosphere (particularly lead) and in leaves collected in the same sampling areas.</style></abstract><notes><style face="normal" font="default" size="100%">doi: 10.1080/03067318908026901</style></notes><research-notes><style face="normal" font="default" size="100%">doi: 10.1080/03067318908026901</style></research-notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Valerio, F.</style></author><author><style face="normal" font="default" size="100%">Brescianini, C.</style></author><author><style face="normal" font="default" size="100%">Lastraioli, S.</style></author><author><style face="normal" font="default" size="100%">Coccia, S.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Metals in Leaves as Indicators of Atmospheric Pollution in Urban Areas</style></title><secondary-title><style face="normal" font="default" size="100%">International Journal of Environmental Analytical Chemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">atmospheric pollution</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy metals (PG)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex leaves</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1989</style></year><pub-dates><date><style  face="normal" font="default" size="100%">1989///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1080/03067318908026901</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">37</style></volume><pages><style face="normal" font="default" size="100%">245 - 251</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Abstract The use of biological indicators to monitor atmospheric pollution is widely distributed. The analysis of heavy metals in leaves is suggested as a method for a rapid identification of polluted areas. Reproducibility and representativity of this method must be assessed. We have verified if the concentration of lead, chromium, copper, nickel and manganese in ?Quercus Ilex? leaves may be used to classify urban areas according to the level of their atmospheric pollution. Particular emphasis was placed on reproducibility of sampling and the analytical method. Preliminary results show a linear correlation between concentration of metals in atmosphere (particularly lead) and in leaves collected in the same sampling areas.</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><notes><style face="normal" font="default" size="100%">doi: 10.1080/03067318908026901doi: 10.1080/03067318908026901The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Taylor &amp; Francis</style></notes></record></records></xml>