<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alvarado, P.</style></author><author><style face="normal" font="default" size="100%">Manjon, J. L.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A quantitative and molecular examination of Tuber melanosporum mycorrhizae in Quercus ilex seedlings from different suppliers in Spain</style></title><secondary-title><style face="normal" font="default" size="100%">FOREST SYSTEMS</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Certification</style></keyword><keyword><style  face="normal" font="default" size="100%">fungi</style></keyword><keyword><style  face="normal" font="default" size="100%">mycorrhiza</style></keyword><keyword><style  face="normal" font="default" size="100%">TRFLP</style></keyword><keyword><style  face="normal" font="default" size="100%">truffle</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">22</style></volume><pages><style face="normal" font="default" size="100%">159 - 169</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Aim of study: The aim of the work was to determine the degree of mycorrhization of Quercus ilex L. subsp. ballota (Desf.) Samp. by the black truffle fungus T. melanosporum Vittad. by quantitative and molecular analyses. Area of study: seedlings inoculated by different Spanish suppliers. Material and methods: The internal transcribed spacers (ITS) of mycorrhizae from different plants were amplified by nested PCR involving fluorescently-labelled primers, and the amplicons either directly sized by ARISA or analysed by TRFLP following their digestion with restriction endonucleases. TRFLP analysis distinguished between mycorrhizae of T melanosporum, T. indicum Cooke &amp; Massee and T. borchii Vittad., as suggested possible by virtual (in silico) TRFLP analysis and real TRFLP analysis of the ascomata of these species. Main results: Significant differences between suppliers were detected in terms of the mean number of mycorrhizae established per plant and percentage mycorrhization. These results allowed the following quality standards for 2 year-old plants to be proposed: a) good quality: &gt;3,000 mycorrhizae/plant, &gt;40% mycorrhization, b) medium (acceptable): &gt;3,000 mycorrhizae/plant, &gt;30% mycorrhization, c) low quality: &lt;3,000 mycorrhizae/plant or &lt;30% mycorrhization, always supposing the mycorrhizae counted represent the species of interest as confirmed by the presence of its DNA and the absence of DNA belonging to contaminating species. Finally, a new microsatellite allelic map obtained from the analysis of several T melanosporum populations across Spain was used to provide a tool capable of determining the geographic origin of the fungi used to inoculate plants. Research highlights: The proposed quality standards can be useful for the evaluation and certification of commercialized Q. ilex plants mycorrhized with T. melanosporum.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;pub-location: CTRA CORUNA KM 7 5, MADRID, 28040, SPAIN&lt;br/&gt;publisher: INST NACIONAL INVESTIGACION TECHNOLOGIA AGRARIA ALIMENTARIA</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Domenech, Jezabel</style></author><author><style face="normal" font="default" size="100%">Ramos-Solano, Beatriz</style></author><author><style face="normal" font="default" size="100%">Probanza, Agustin</style></author><author><style face="normal" font="default" size="100%">Lucas-Garcı́a, José Antonio</style></author><author><style face="normal" font="default" size="100%">Colón, Juan José</style></author><author><style face="normal" font="default" size="100%">Gutiérrez-Mañero, Francisco Javier</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Bacillus spp. and Pisolithus tinctorius effects on Quercus ilex ssp. ballota: a study on tree growth, rhizosphere community structure and mycorrhizal infection</style></title><secondary-title><style face="normal" font="default" size="100%">Forest Ecology and Management</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ballota</style></keyword><keyword><style  face="normal" font="default" size="100%">microbial community structure</style></keyword><keyword><style  face="normal" font="default" size="100%">mycorrhiza</style></keyword><keyword><style  face="normal" font="default" size="100%">PGPR</style></keyword><keyword><style  face="normal" font="default" size="100%">quercus ilex ssp</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2004</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2004///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://linkinghub.elsevier.com/retrieve/pii/S0378112704001501</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">194</style></volume><pages><style face="normal" font="default" size="100%">293 - 303</style></pages><isbn><style face="normal" font="default" size="100%">3491351049</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A local species of oak (Quercus ilex ssp. ballota) was inoculated or co-inoculated with the ectomycorrhizal fungus Pisolithus tinctorius and two plant growth promoting rhizobacteria (PGPR) of the genus Bacillus (Bacillus licheniformis CECT 5106 and Bacillus pumilus CECT 5105). Effects of inoculation on growth, on N acquisition by the plant roots, changes in rhizosphere microbial communities and the degree of mycorrhization were evaluated. Only B. licheniformis promoted the growth of Q. ilex seedlings while co-inoculation of either bacterial strain with P. tinctorius had a negative effect on plant growth. Furthermore, B. licheniformis inhibited fungal growth as revealed by ergosterol/chitin analysis. As shown by phospholipid fatty acid proﬁles, the inoculation caused a slight alteration in the microbial community structure of the rhizosphere, both in the total community and the culturable populations.</style></abstract><issue><style face="normal" font="default" size="100%">1-3</style></issue></record></records></xml>