<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vernesi, Cristiano</style></author><author><style face="normal" font="default" size="100%">Rocchini, Duccio</style></author><author><style face="normal" font="default" size="100%">Pecchioli, Elena</style></author><author><style face="normal" font="default" size="100%">Neteler, Markus</style></author><author><style face="normal" font="default" size="100%">Vendramin, Giovanni G</style></author><author><style face="normal" font="default" size="100%">Paffetti, Donatella</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A landscape genetics approach reveals ecological-based differentiation in populations of holm oak (Quercus ilex L.) at the northern limit of its range</style></title><secondary-title><style face="normal" font="default" size="100%">Biological Journal of the Linnean Society</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bayesian computation</style></keyword><keyword><style  face="normal" font="default" size="100%">Geneland</style></keyword><keyword><style  face="normal" font="default" size="100%">Italian Prealps</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><volume><style face="normal" font="default" size="100%">107</style></volume><pages><style face="normal" font="default" size="100%">458-467</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The holm oak plays a relevant role in the functioning of Mediterranean forests. In the area north of Garda Lake, Italian Prealps, holm oak populations are at the northernmost edge of their distribution. Being peripheral, these populations are of particular interest for ecological, evolutionary and conservation studies. Through an explicit individual-based landscape genetics approach, we addressed the following questions: (1) are levels of genetic variation reduced in these marginal populations compared with central populations?; (2) despite the narrow geographical scale, do individual-based analyses have some power to detect genetic differentiation?; (3) do environmental and/or climatic factors exert a role in shaping patterns of genetic variation and differentiation? Through a Bayesian method, we identified three clusters whose genetic variability can be considered to be of the same order as that recorded in central Quercus ilex populations. Although being geographically very close (&lt; 20 km), the differentiation was statistically significant (P &lt; 0.05) with global F</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Burgarella, C.</style></author><author><style face="normal" font="default" size="100%">Lorenzo, Z.</style></author><author><style face="normal" font="default" size="100%">Jabbour-Zahab, R.</style></author><author><style face="normal" font="default" size="100%">Lumaret, R.</style></author><author><style face="normal" font="default" size="100%">Guichoux, E.</style></author><author><style face="normal" font="default" size="100%">Petit, R. J.</style></author><author><style face="normal" font="default" size="100%">Soto, A.</style></author><author><style face="normal" font="default" size="100%">Gil, L.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection of hybrids in nature: application to oaks (Quercus suber and Q. ilex).</style></title><secondary-title><style face="normal" font="default" size="100%">Heredity</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">clustering analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">introgressive hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword><keyword><style  face="normal" font="default" size="100%">Q. ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Simulation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2009///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19240752</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">102</style></volume><pages><style face="normal" font="default" size="100%">442 - 452</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Powerful and accurate detection of first-generation (F1) hybrids and backcrosses in nature is needed to achieve a better understanding of the function and dynamics of introgression. To document the frequency of ongoing interspecific gene exchange between two Mediterranean evergreen oaks, the cork oak (Quercus suber) and the holm oak (Q. ilex), we analyzed 1487 individuals originating from across the range of the two species using eight microsatellite loci and two Bayesian clustering approaches (implemented in the programs STRUCTURE and NEWHYBRIDS). Simulated data were used to assess the differences between the two clustering methods and to back up the choice of the threshold value for the posterior probability to discriminate admixed from pure individuals. We found that the use of STRUCTURE resulted in the highest power to detect hybrids, whereas NEWHYBRIDS provided the highest accuracy. Irrespective of the approach, the two species were clearly distinguished as independent genetic entities without any prior information. In contrast with previous reports, we found no evidence for unidirectional introgression. The overall hybridization rate was very low (&lt;2% of introgressed individuals). Only two individuals were identified as F1 hybrids and five as early backcrosses. This work shows that the combined application of the two complementary Bayesian approaches and their systematic validation with simulations, fit for the case at hand, helps gain resolution in the identification of admixed individuals.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 19240752</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Burgarella, C</style></author><author><style face="normal" font="default" size="100%">Lorenzo, Z</style></author><author><style face="normal" font="default" size="100%">Jabbour-Zahab, R</style></author><author><style face="normal" font="default" size="100%">Lumaret, R</style></author><author><style face="normal" font="default" size="100%">Guichoux, E</style></author><author><style face="normal" font="default" size="100%">Petit, R J</style></author><author><style face="normal" font="default" size="100%">Soto, Á</style></author><author><style face="normal" font="default" size="100%">Gil, L</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Detection of hybrids in nature: application to oaks (Quercus suber and Q. ilex).</style></title><secondary-title><style face="normal" font="default" size="100%">Heredity</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">clustering analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">introgressive hybridization</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword><keyword><style  face="normal" font="default" size="100%">Q. ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Simulation</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year></dates><volume><style face="normal" font="default" size="100%">102</style></volume><pages><style face="normal" font="default" size="100%">442-452</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Powerful and accurate detection of first-generation (F1) hybrids and backcrosses in nature is needed to achieve a better understanding of the function and dynamics of introgression. To document the frequency of ongoing interspecific gene exchange between two Mediterranean evergreen oaks, the cork oak (Quercus suber) and the holm oak (Q. ilex), we analyzed 1487 individuals originating from across the range of the two species using eight microsatellite loci and two Bayesian clustering approaches (implemented in the programs STRUCTURE and NEWHYBRIDS). Simulated data were used to assess the differences between the two clustering methods and to back up the choice of the threshold value for the posterior probability to discriminate admixed from pure individuals. We found that the use of STRUCTURE resulted in the highest power to detect hybrids, whereas NEWHYBRIDS provided the highest accuracy. Irrespective of the approach, the two species were clearly distinguished as independent genetic entities without any prior information. In contrast with previous reports, we found no evidence for unidirectional introgression. The overall hybridization rate was very low (&lt;2% of introgressed individuals). Only two individuals were identified as F1 hybrids and five as early backcrosses. This work shows that the combined application of the two complementary Bayesian approaches and their systematic validation with simulations, fit for the case at hand, helps gain resolution in the identification of admixed individuals.</style></abstract><accession-num><style face="normal" font="default" size="100%">19240752</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Hornero, J</style></author><author><style face="normal" font="default" size="100%">Gallego, F J</style></author><author><style face="normal" font="default" size="100%">Martinez, I</style></author><author><style face="normal" font="default" size="100%">TORIBIO, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Testing the conservation of Quercus spp. microsatellites in the cork oak, Q-suber L.</style></title><secondary-title><style face="normal" font="default" size="100%">SILVAE GENETICA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cork oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Fagaceae</style></keyword><keyword><style  face="normal" font="default" size="100%">microsatellites</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">J D SAUERLANDERS VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">FINKENHOFSTRASSE 21, D-60322 FRANKFURT, GERMANY</style></pub-location><volume><style face="normal" font="default" size="100%">50</style></volume><pages><style face="normal" font="default" size="100%">162-167</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The transferability of microsatellite (SSR) loci from Quercus spp. to cork oak (Quercus suber L.) was investigated. Semi-automated analysis of fluorescently-labelled PCR fragments was used to test 24 primer sets developed for Q. myrsinifolia BLUME, Q. petraea (MAT7.) LIEB. and Q. robur L. in 41 cork oak trees from four stands covering the main area of distribution of the species in Spain. Successful cross-species events occurred for 13 loci (54%). Two of them were monomorphic and another two appeared as multilocus. High levels of genetic variability were detected both for the number of alleles, 62 (7.5 per polymorphic locus, with a maximum number of 19 in locus ssrQpZAG110) and for the expected heterozygosity (mean H-E = 0.648). These results were much higher than those previously reported by other authors using allozyme loci. The usefulness of the. SSR loci successfully amplified for studies on population genetics of cork oak is discussed.</style></abstract></record></records></xml>