<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Llusia, Joan</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Munné-Bosch, Sergi</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Sustained accumulation of methyl salicylate alters antioxidant protection and reduces tolerance of holm oak to heat stress</style></title><secondary-title><style face="normal" font="default" size="100%">Physiologia Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">antioxidant protection (voyant)</style></keyword><keyword><style  face="normal" font="default" size="100%">foliar MeSA accumulation</style></keyword><keyword><style  face="normal" font="default" size="100%">heat stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">methyl salicylate</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1111/j.1399-3054.2005.00519.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">124</style></volume><pages><style face="normal" font="default" size="100%">353 - 361</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Methyl salicylate (MeSA) is thought to have a major role in biotic and abiotic stresses by acting as a signal to trigger the oxidative burst, which is needed to activate gene expression in plant stress responses. To assess the potential effects of sustained foliar accumulation of MeSA on plant stress tolerance, the extent of photo- and antioxidant protection, lipid peroxidation and visual leaf area damage were evaluated in MeSA-treated (c. 60 nl l−1 in air) and control holm oak (Quercus ilex L.) plants exposed to heat stress. Control plants showed an increase in foliar MeSA levels up to 1.8 nmol [gDW]−1 as temperature increased and they displayed tolerance to temperatures as high as 45°C, which might be attributed, at least in part, to enhanced xanthophyll de-epoxidation and increases in ascorbate and α-tocopherol. MeSA-treated plants showed a sustained foliar accumulation of this compound, with values ranging from 10 to 23 nmol [gDW]−1 throughout the experiment. These plants showed lower ascorbate and tocopherol levels and higher oxidative damage at 50°C than controls, as indicated by enhanced malondialdehyde levels and leaf area damage and lower maximum efficiency of PSII photochemistry (Fv/Fm ratio). These results demonstrate that a sustained foliar accumulation of MeSA is detrimental to plant function and that it can reduce thermotolerance in holm oak by altering antioxidant defences.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Munksgaard International Publishers</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Jofre, A</style></author><author><style face="normal" font="default" size="100%">Molinas, M</style></author><author><style face="normal" font="default" size="100%">Pla, M</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">A 10-kDa class-CI sHsp protects E-coli from oxidative and high-temperature stress</style></title><secondary-title><style face="normal" font="default" size="100%">PLANTA</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">chaperone activity</style></keyword><keyword><style  face="normal" font="default" size="100%">heat stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxidative stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">small heat-shock protein</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year></dates><publisher><style face="normal" font="default" size="100%">SPRINGER-VERLAG</style></publisher><pub-location><style face="normal" font="default" size="100%">175 FIFTH AVE, NEW YORK, NY 10010 USA</style></pub-location><volume><style face="normal" font="default" size="100%">217</style></volume><pages><style face="normal" font="default" size="100%">813-819</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">We report on a new cDNA clone (Qshsp10.4-CI) of a Quercus suber L. class-CI small heat-shock protein (sHsp) obtained from cork (phellem), a highly oxidatively stressed plant tissue. The deduced gene product lacks the C-terminal extension and the consensus I region of the alpha-crystallin domain, being the most C-terminally truncated sHsp reported to date. In an attempt to prove that a protective function is possible for such a truncated sHsp, we overexpressed in Escherichia coli three recombinant sHsp-CIs, one (rQsHsp10.4-CI) showing the same truncation as Qshsp10.4-CI, a second (rN49) lacking the whole alpha-crystallin domain, and a third (rN153) consisting of a full-length sHsp-CI. The overexpression of rN153 and, remarkably, rQsHsp10.4-CI but not rN49 enhanced cell viability under high temperature and, interestingly, under oxidative stress. These results show that the C-terminal extension and the consensus I region of the alpha-crystallin domain are dispensable, but amino acids 1-41 of the alpha-crystallin domain (including the consensus II region) are essential for the protective activity of sHsp-CIs. On the other hand, two-dimensional immunodetection patterns showed accumulation of ca. 10-kDa sHsp-CI immunorelated polypeptides in cork and other oxidatively stressed tissues but not in control and heat-stressed tissues. We discuss the possible role of highly truncated sHsps in relation to oxidative stress.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Staudt, M</style></author><author><style face="normal" font="default" size="100%">Bertin, N</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Light and temperature dependence of the emission of cyclic and acyclic monoterpenes from holm oak (Quercus ilex L.) leaves</style></title><secondary-title><style face="normal" font="default" size="100%">Plant, Cell &amp; Environment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">biogenic VOC emission</style></keyword><keyword><style  face="normal" font="default" size="100%">emission model</style></keyword><keyword><style  face="normal" font="default" size="100%">heat stress</style></keyword><keyword><style  face="normal" font="default" size="100%">Holm oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Isoprenoids</style></keyword><keyword><style  face="normal" font="default" size="100%">light effect</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">temperature effect</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1998</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Publishing Ltd</style></publisher><volume><style face="normal" font="default" size="100%">21</style></volume><pages><style face="normal" font="default" size="100%">385-395</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">In a laboratory study, we investigated the monoterpene emissions from Quercus ilex, an evergreen sclerophyllous Mediterranean oak species whose emissions are light dependent. We examined the light and temperature responses of individual monoterpenes emitted from leaves under various conditions, the effect of heat stress on emissions, and the emission-onset during leaf development. Emission rate increased 10-fold during leaf growth, with slight changes in the composition. At 30 °C and saturating light, the monoterpene emission rate from mature leaves averaged 4·1 nmol m–2 s–1, of which α-pinene, sabinene and β-pinene accounted for 85%. The light dependence of emission was similar for all monoterpenes: it resembled the light saturation curve of CO2 assimilation, although monoterpene emission continued in the dark. Temperature dependence differed among emitted compounds: most of them exhibited an exponential increase up to 35 °C, a maximum at 42 °C, and a slight decline at higher temperatures. However, the two acyclic isomers cis-β-ocimene and trans-β-ocimene were hardly detected below 35 °C, but their emission rates increased above this temperature as the emission rates of other compounds fell, so that total emission of monoterpenes exponentially increased from 5 to 45 °C. The ratio between ocimene isomers and other compounds increased with both absolute temperature and time of heat exposure. The light dependence of emission was insensitive to the temperature at which it was measured, and vice versa the temperature dependence was insensitive to the light regime. The results demonstrated that none of the models currently applied to simulate isoprene or monoterpene emissions correctly predicts the short-term effects of light and temperature on Q. ilex emissions. The percentage of fixed carbon lost immediately as monoterpenes ranged between 0·1 and 6·0% depending on temperature, but rose up to 20% when leaves were continuously exposed to temperatures between 40 and 45 °C.</style></abstract></record></records></xml>