<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vidal, Nieves</style></author><author><style face="normal" font="default" size="100%">Viéitez, Ana M</style></author><author><style face="normal" font="default" size="100%">Fernández, M Rosario</style></author><author><style face="normal" font="default" size="100%">Cuenca, Beatriz</style></author><author><style face="normal" font="default" size="100%">Ballester, Antonio</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Establishment of cryopreserved gene banks of European chestnut and cork oak</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Forest Research</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Castanea sativa</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryopreservation</style></keyword><keyword><style  face="normal" font="default" size="100%">forest tree species</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid nitrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">Vitrification</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year></dates><volume><style face="normal" font="default" size="100%">129</style></volume><pages><style face="normal" font="default" size="100%">635-643</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cryopreservation of selected genotypes of European chestnut and cork oak was carried out in two laboratories in a project involving conservation of ﬁeld collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised of vitriﬁcation of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty-three out of 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embryogenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to those derived from nonsupercooled material. In light of the results obtained, longterm cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during ﬁeld evaluation</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Gonzalez-Benito, M Elena</style></author><author><style face="normal" font="default" size="100%">Prieto, Roberto-Moreno</style></author><author><style face="normal" font="default" size="100%">Herradon, Esther</style></author><author><style face="normal" font="default" size="100%">Martin, Carmen</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Cryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors.</style></title><secondary-title><style face="normal" font="default" size="100%">Cryo letters</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Cryopreservation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryopreservation: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryoprotective Agents</style></keyword><keyword><style  face="normal" font="default" size="100%">Cryoprotective Agents: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Culture Techniques: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Desiccation</style></keyword><keyword><style  face="normal" font="default" size="100%">Desiccation: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">embryonic axes</style></keyword><keyword><style  face="normal" font="default" size="100%">germination</style></keyword><keyword><style  face="normal" font="default" size="100%">germination temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Germination: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">liquid nitrogen</style></keyword><keyword><style  face="normal" font="default" size="100%">plantlet development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Seeds: embryology</style></keyword><keyword><style  face="normal" font="default" size="100%">Survival Rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year></dates><volume><style face="normal" font="default" size="100%">23</style></volume><pages><style face="normal" font="default" size="100%">283-290</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This study examines different factors included in the cryopreservation protocols for Quercus ilex and Q. suber embryonic axes. In vitro incubation temperature played an important role in the appropriate development of Q. ilex axes, as 15 degrees C was superior to 25 degrees C. Q. suber axes proved to be more sensitive to desiccation and cooling. Poor survival (35%) was observed when axes were included into cryovials and then in liquid nitrogen, and none when immersed in sub-cooled liquid nitrogen (-210 degrees C). Q. ilex axes showed poorly organised development in vitro (c. 50% of non-cooled axes showed shoot development). However, c. 80% survival was observed after cryopreservation (either in liquid nitrogen or sub-cooled liquid nitrogen at 0.34 g water / g dry weight), of which c. 15% showed shoot development.</style></abstract><accession-num><style face="normal" font="default" size="100%">12447487</style></accession-num></record></records></xml>