<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Correia, Barbara</style></author><author><style face="normal" font="default" size="100%">Valledor, Luis</style></author><author><style face="normal" font="default" size="100%">Meijón, Mónica</style></author><author><style face="normal" font="default" size="100%">Rodriguez, José Luis</style></author><author><style face="normal" font="default" size="100%">Dias, Maria Celeste</style></author><author><style face="normal" font="default" size="100%">Santos, Conceição</style></author><author><style face="normal" font="default" size="100%">Cañal, Maria Jesus</style></author><author><style face="normal" font="default" size="100%">Rodriguez, Roberto</style></author><author><style face="normal" font="default" size="100%">Pinto, Glória</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?</style></title><secondary-title><style face="normal" font="default" size="100%">PLoS ONE</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Acclimatization</style></keyword><keyword><style  face="normal" font="default" size="100%">Acclimatization: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Acetylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Blotting</style></keyword><keyword><style  face="normal" font="default" size="100%">CHROMATIN</style></keyword><keyword><style  face="normal" font="default" size="100%">CORK oak (citation</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine: analogs &amp; derivatives</style></keyword><keyword><style  face="normal" font="default" size="100%">Deoxycytidine: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA methylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrolytes</style></keyword><keyword><style  face="normal" font="default" size="100%">Electrolytes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Epigenesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene expression</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic Markers</style></keyword><keyword><style  face="normal" font="default" size="100%">Genome</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat-Shock Response</style></keyword><keyword><style  face="normal" font="default" size="100%">Heat-Shock Response: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Histones</style></keyword><keyword><style  face="normal" font="default" size="100%">Histones: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological</style></keyword><keyword><style  face="normal" font="default" size="100%">Physiological: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">POST-translational modification</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Random Amplified Polymorphic DNA Technique</style></keyword><keyword><style  face="normal" font="default" size="100%">Stress</style></keyword><keyword><style  face="normal" font="default" size="100%">tags)</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">TREES -- Research</style></keyword><keyword><style  face="normal" font="default" size="100%">Western</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2013</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2013///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3543447&amp;tool=pmcentrez&amp;rendertype=abstracthttp://dx.doi.org/10.1371/journal.pone.0053543</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">8</style></volume><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Trees necessarily experience changes in temperature, requiring efficient short-term strategies that become crucial in environmental change adaptability. DNA methylation and histone posttranslational modifications have been shown to play a key role in both epigenetic control and plant functional status under stress by controlling the functional state of chromatin and gene expression. Cork oak (Quercus suber L.) is a key stone of the Mediterranean region, growing at temperatures of 45°C. This species was subjected to a cumulative temperature increase from 25°C to 55°C under laboratory conditions in order to test the hypothesis that epigenetic code is related to heat stress tolerance. Electrolyte leakage increased after 35°C, but all plants survived to 55°C. DNA methylation and acetylated histone H3 (AcH3) levels were monitored by HPCE (high performance capillary electrophoresis), MS-RAPD (methylation-sensitive random-amplified polymorphic DNA) and Protein Gel Blot analysis and the spatial distribution of the modifications was assessed using a confocal microscope. DNA methylation analysed by HPCE revealed an increase at 55°C, while MS-RAPD results pointed to dynamic methylation-demethylation patterns over stress. Protein Gel Blot showed the abundance index of AcH3 decreasing from 25°C to 45°C. The immunohistochemical detection of 5-mC (5-methyl-2′-deoxycytidine) and AcH3 came upon the previous results. These results indicate that epigenetic mechanisms such as DNA methylation and histone H3 acetylation have opposite and particular dynamics that can be crucial for the stepwise establishment of this species into such high stress (55°C), allowing its acclimation and survival. This is the first report that assesses epigenetic regulation in order to investigate heat tolerance in forest trees.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Public Library of Science&lt;br/&gt;accession-num: 23326451</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">De Nicola, F.</style></author><author><style face="normal" font="default" size="100%">Maisto, G.</style></author><author><style face="normal" font="default" size="100%">Prati, M. V.</style></author><author><style face="normal" font="default" size="100%">Alfani, A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Leaf accumulation of trace elements and polycyclic aromatic hydrocarbons (PAHs) in Quercus ilex L.</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental pollution (Barking, Essex : 1987)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Air Pollutants</style></keyword><keyword><style  face="normal" font="default" size="100%">Air Pollutants: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Aromatic</style></keyword><keyword><style  face="normal" font="default" size="100%">Aromatic: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Atomic</style></keyword><keyword><style  face="normal" font="default" size="100%">biomonitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">cadmium</style></keyword><keyword><style  face="normal" font="default" size="100%">Cadmium: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">chromium</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromium: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cities</style></keyword><keyword><style  face="normal" font="default" size="100%">copper</style></keyword><keyword><style  face="normal" font="default" size="100%">Copper: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Iron</style></keyword><keyword><style  face="normal" font="default" size="100%">Iron: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Italy</style></keyword><keyword><style  face="normal" font="default" size="100%">lead</style></keyword><keyword><style  face="normal" font="default" size="100%">Lead: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">PAHs</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Polycyclic Hydrocarbons</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex L.</style></keyword><keyword><style  face="normal" font="default" size="100%">Spectrophotometry</style></keyword><keyword><style  face="normal" font="default" size="100%">Trace Elements</style></keyword><keyword><style  face="normal" font="default" size="100%">Trace Elements: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Unwashed and washed leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Vanadium</style></keyword><keyword><style  face="normal" font="default" size="100%">Vanadium: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Zinc</style></keyword><keyword><style  face="normal" font="default" size="100%">Zinc: analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17892907</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">153</style></volume><pages><style face="normal" font="default" size="100%">376 - 383</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Quercus ilex L. leaves were collected four times in one year at six urban sites and one remote area in order to determine trace element and PAH accumulation through concomitant analyses of unwashed and water-washed leaves. Both unwashed and washed leaves showed the highest amounts of trace elements and PAHs in the urban area. Unwashed leaves showed greater differences between urban and remote areas and among the urban sites than washed leaves for trace element and PAH concentrations. Water-washing resulted in a significant (P&lt;0.001) decrease in leaf concentrations of Cr, Cu, Fe, Pb, V and Zn. By contrast, Cd and total PAH concentrations showed no differences between unwashed and washed leaves.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 17892907</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palacio, Sara</style></author><author><style face="normal" font="default" size="100%">Milla, Rubén</style></author><author><style face="normal" font="default" size="100%">Albuixech, Jorge</style></author><author><style face="normal" font="default" size="100%">Pérez-Rontomé, Carmen</style></author><author><style face="normal" font="default" size="100%">Camarero, Jesús Julio</style></author><author><style face="normal" font="default" size="100%">Maestro, Melchor</style></author><author><style face="normal" font="default" size="100%">Montserrat-Martí, Gabriel</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Seasonal variability of dry matter content and its relationship with shoot growth and nonstructural carbohydrates</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbohydrate Metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">functional classifications</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf dry matter content (LDMC)</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf traits</style></keyword><keyword><style  face="normal" font="default" size="100%">leaf water status</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenology</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Stems: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants</style></keyword><keyword><style  face="normal" font="default" size="100%">Plants: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasons</style></keyword><keyword><style  face="normal" font="default" size="100%">shoot growth</style></keyword><keyword><style  face="normal" font="default" size="100%">Species Specificity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/18643937http://dx.doi.org/10.1111/j.1469-8137.2008.02569.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">180</style></volume><pages><style face="normal" font="default" size="100%">133 - 142</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • This study assesses how different phases of shoot growth underlie seasonal change in leaf and stem dry matter content (LDMC and SDMC, respectively) of 12 woody Mediterranean species. The relationship between LDMC and nonstructural carbohydrate (NSC) concentrations is also explored and the seasonal vs interspecies variability of LDMC compared. * • LDMC, SDMC and shoot elongation rate (SER) were measured on a monthly basis for a minimum of 12 months. Bud growth rate (BGR) and NSC concentrations were also assessed in several of the study species. * • LDMC and SDMC decreased during shoot elongation in spring and increased in summer, showing a significant negative correlation with SER, but were unrelated to BGR. Half of the species analysed showed a positive relationship between LDMC and NSC. Seasonal fluctuations of LDMC within species were higher than interspecies differences, and species ranking was significantly affected by the month of sampling, except during winter months. * • Seasonal changes in LDMC and SDMC are mainly related to shoot elongation phenology, and NSC sink–source relationships between old and growing organs can explain this relationship in some species. Owing to the high seasonal variability in LDMC, it is recommended that samples for comparative purposes should be collected as close to the winter as possible.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Publishing Ltd&lt;br/&gt;accession-num: 18643937</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Domínguez, María T.</style></author><author><style face="normal" font="default" size="100%">Marañón, Teodoro</style></author><author><style face="normal" font="default" size="100%">Murillo, José M.</style></author><author><style face="normal" font="default" size="100%">Schulin, Rainer</style></author><author><style face="normal" font="default" size="100%">Robinson, Brett H.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Trace element accumulation in woody plants of the Guadiamar Valley, SW Spain: a large-scale phytomanagement case study.</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental pollution (Barking, Essex : 1987)</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Bioaccumulation</style></keyword><keyword><style  face="normal" font="default" size="100%">biodegradation</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy metal</style></keyword><keyword><style  face="normal" font="default" size="100%">Heavy: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Mass spectrometry</style></keyword><keyword><style  face="normal" font="default" size="100%">metals</style></keyword><keyword><style  face="normal" font="default" size="100%">Mining</style></keyword><keyword><style  face="normal" font="default" size="100%">Olea</style></keyword><keyword><style  face="normal" font="default" size="100%">Olea europaea</style></keyword><keyword><style  face="normal" font="default" size="100%">Olea: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Phytoremediation</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Populus</style></keyword><keyword><style  face="normal" font="default" size="100%">Populus alba</style></keyword><keyword><style  face="normal" font="default" size="100%">Populus: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil Pollutants</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil Pollutants: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Species Specificity</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17602809</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">152</style></volume><pages><style face="normal" font="default" size="100%">50 - 59</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Phytomanagement employs vegetation and soil amendments to reduce the environmental risk posed by contaminated sites. We investigated the distribution of trace elements in soils and woody plants from a large phytomanaged site, the Guadiamar Valley (SW Spain), 7 years after a mine spill, which contaminated the area in 1998. At spill-affected sites, topsoils (0-25 cm) had elevated concentrations of As (129 mg kg(-1)), Bi (1.64 mg kg(-1)), Cd (1.44 mg kg(-1)), Cu (115 mg kg(-1)), Pb (210 mg kg(-1)), Sb (13.8 mg kg(-1)), Tl (1.17 mg kg(-1)) and Zn (457 mg kg(-1)). Trace element concentrations in the studied species were, on average, within the normal ranges for higher plants. An exception was white poplar (Populus alba), which accumulated Cd and Zn in leaves up to 3 and 410 mg kg(-1) respectively. We discuss the results with regard to the phytomanagement of trace element contaminated sites.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 17602809</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Filella, Iolanda</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author><author><style face="normal" font="default" size="100%">Llusia, Joan</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Dynamics of the enhanced emissions of monoterpenes and methyl salicylate, and decreased uptake of formaldehyde, by Quercus ilex leaves after application of jasmonic acid</style></title><secondary-title><style face="normal" font="default" size="100%">New Phytologist</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Carbon dioxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Carbon Dioxide: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclopentanes</style></keyword><keyword><style  face="normal" font="default" size="100%">Cyclopentanes: pharmacology</style></keyword><keyword><style  face="normal" font="default" size="100%">Dehydration</style></keyword><keyword><style  face="normal" font="default" size="100%">Formaldehyde</style></keyword><keyword><style  face="normal" font="default" size="100%">Formaldehyde: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">jasmonic acid (JA)</style></keyword><keyword><style  face="normal" font="default" size="100%">light</style></keyword><keyword><style  face="normal" font="default" size="100%">methyl salicylate</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes</style></keyword><keyword><style  face="normal" font="default" size="100%">Monoterpenes: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">net photosynthetic rates</style></keyword><keyword><style  face="normal" font="default" size="100%">Oxylipins</style></keyword><keyword><style  face="normal" font="default" size="100%">photosynthesis</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex (holm oak)</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Salicylates</style></keyword><keyword><style  face="normal" font="default" size="100%">Salicylates: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Stomatal conductance</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">VOC (volatile organic compound)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16390425http://dx.doi.org/10.1111/j.1469-8137.2005.01570.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">169</style></volume><pages><style face="normal" font="default" size="100%">135 - 144</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">* • Jasmonic acid (JA) is a signalling compound with a key role in both stress and development in plants, and is reported to elicit the emission of volatile organic compounds (VOCs). Here we studied the dynamics of such emissions and the linkage with photosynthetic rates and stomatal conductance. * • We sprayed JA on leaves of the Mediterranean tree species Quercus ilex and measured the photosynthetic rates, stomatal conductances, and emissions and uptake of VOCs using proton transfer reaction mass spectrometry and gas chromatography after a dark–light transition. * • Jasmonic acid treatment delayed the induction of photosynthesis and stomatal conductance by approx. 20 min, and decreased them 24 h after spraying. Indications were found of both stomatal and nonstomatal limitations of photosynthesis. Monoterpene emissions were enhanced (20–30%) after JA spraying. Jasmonic acid also increased methyl salicylate (MeSa) emissions (more than twofold) 1 h after treatment, although after 24 h this effect had disappeared. Formaldehyde foliar uptake decreased significantly 24 h after JA treatment. * • Both biotic and abiotic stresses can thus affect plant VOC emissions through their strong impact on JA levels. Jasmonic acid-mediated increases in monoterpene and MeSa emissions might have a protective role when confronting biotic and abiotic stresses.</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Publishing Ltd&lt;br/&gt;accession-num: 16390425</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Castro-Díez, Pilar</style></author><author><style face="normal" font="default" size="100%">Navarro, Javier</style></author><author><style face="normal" font="default" size="100%">Pintado, Ana</style></author><author><style face="normal" font="default" size="100%">Sancho, Leopoldo G.</style></author><author><style face="normal" font="default" size="100%">Maestro, Melchor</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Interactive effects of shade and irrigation on the performance of seedlings of three Mediterranean Quercus species</style></title><secondary-title><style face="normal" font="default" size="100%">Tree Physiology</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Agriculture</style></keyword><keyword><style  face="normal" font="default" size="100%">Agriculture: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Analysis of Variance</style></keyword><keyword><style  face="normal" font="default" size="100%">biomass allocation</style></keyword><keyword><style  face="normal" font="default" size="100%">carbon assimilation rate</style></keyword><keyword><style  face="normal" font="default" size="100%">fluorescence</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean Region</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenology</style></keyword><keyword><style  face="normal" font="default" size="100%">Phenotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Shoots: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Relative growth rate</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: drug effects</style></keyword><keyword><style  face="normal" font="default" size="100%">Seedling: physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">water</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Water: pharmacology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16356909http://treephys.oxfordjournals.org/content/26/3/389.abstract</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">26</style></volume><pages><style face="normal" font="default" size="100%">389 - 400</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Shade and irrigation are frequently used to increase the success of Mediterranean Quercus spp. plantations. However, there is controversy about the combined effects of these treatments on plant performance. We assessed the effects of two irradiances (full sunlight and moderate shade) and two summer watering regimes (high (daily) and low (alternate days)) on leaf and whole-plant traits of 1-year-old seedlings of Quercus coccifera, Q. ilex subsp. ballota and Q. faginea grown outdoors for 8.5 months. Leaf traits included measures of morphology, nitrogen concentration, gas exchange and photochemical efficiency, and measures of whole-plant traits included biomass allocation patterns, growth phenology, across-summer leaf area change and relative growth rate (RGR).Moderate shade reduced leaf mass per area, increased photochemical efficiency, maximum carbon assimilation rate (Amax) and allocation to leaves, and prolonged the growing period in one or more of the species. Daily watering in summer increased Amax of Q. ilex and prolonged the growing period of Q. ilex and Q. faginea. Both treatments tended to increase RGR. The effect of shade was greater in the low-watering regime than in the high-watering regime for two of the 15 studied traits, with treatment effects being independent for the remaining 13 traits. Leaf nitrogen and the ability to maintain leaf area after the arid period, rather than biomass allocation traits, explained the variation in seedling RGR. Trait responsiveness to the treatments was low and similar among species and between study scales, being unexpectedly low in Q. faginea leaves. This may be because selective pressures on leaf plasticity act differently in deciduous and evergreen species. We conclude that moderate shade and daily summer watering enhance the performance of Mediterranean Quercus seedlings through species-specific mechanisms.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">From Duplicate 2 (Interactive effects of shade and irrigation on the performance of seedlings of three Mediterranean Quercus species - Castro-Díez, Pilar; Navarro, Javier; Pintado, Ana; Sancho, Leopoldo G; Maestro, Melchor)From Duplicate 2 (Interactive effects of shade and irrigation on the performance of seedlings of three Mediterranean Quercus species - Castro-Díez, Pilar; Navarro, Javier; Pintado, Ana; Sancho, Leopoldo G; Maestro, Melchor)The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 16356909</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Sardans, Jordi</style></author><author><style face="normal" font="default" size="100%">Penuelas, Josep</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Introduction of the factor of partitioning in the lithogenic enrichment factors of trace element bioaccumulation in plant tissues.</style></title><secondary-title><style face="normal" font="default" size="100%">Environmental monitoring and assessment</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">biomass</style></keyword><keyword><style  face="normal" font="default" size="100%">Bryopsida</style></keyword><keyword><style  face="normal" font="default" size="100%">Bryopsida: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Bryopsida: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring: methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: growth &amp; development</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil Pollutants</style></keyword><keyword><style  face="normal" font="default" size="100%">Soil Pollutants: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Spain</style></keyword><keyword><style  face="normal" font="default" size="100%">Trace Elements</style></keyword><keyword><style  face="normal" font="default" size="100%">Trace Elements: analysis</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2006///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16648953</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">115</style></volume><pages><style face="normal" font="default" size="100%">473 - 98</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Bioindicators are widely used in the study of trace elements inputs into the environment and great efforts have been conducted to separate atmospheric from soil borne inputs on biomass accumulation. Many monitoring studies of trace element pollution take into account the dust particles located in the plant surface plus the contents of the plant tissues. However, it is usually only the trace element content in the plant tissues that is relevant on plant health. Enrichment factor equations take into account the trace element enrichment of biomasses with respect soil or bedrocks by comparing the ratios of the trace element in question to a lithogenic element, usually Al. However, the enrichment equations currently in use are inadequate because they do not take into account the fact that Al (or whichever reference element) and the element in question may have different solubility-absorption-retention levels depending on the rock and soil types involved. This constrain will become critical when results from different sites are compared and so in this article we propose that the solubility factors of each element are taken into account in order to overcome this constrain. We analysed Sb, Co, Ni, Cr, Pb, Cd, Mn, V, Zn, Cu, As, Hg, and Al concentration in different zones of Catalonia (NE Spain) using the evergreen oak Quercus ilex and the moss Hypnum cupressiforme as target species. We compared the results obtained in rural and non industrial areas with those from the Barcelona Metropolitan Area. We observed differences in Al concentrations of soils and bedrocks at each different site, together with the differences in solubility between Al and the element in question, and a weak correlation between total soil content and water extract content through different sites for most trace elements. All these findings show the unsuitability of the current enrichment factors for calculating lithospheric and atmospheric contributions to trace element concentrations in biomass tissues. The trace element enrichment factors were calculated by subtracting the part predicted by substrate composition (deduced from water extracts from soils and bedrock) from total concentrations. Results showed that for most of the trace elements analysed, trace elements enrichment factors were higher inside the Barcelona Metropolitan Area than outside, a finding that indicates that greater atmospheric inputs occur in urban areas. The results show that the most useful and correct way of establishing a reference for lithospheric and atmospheric inputs into the plant tissues is, first, to analyse samples of the same plant species collected from a number of sites possessing similar environmental conditions (climate, vegetation type, soil type) and, second, to use this new enrichment factor obtained by subtracting from the total concentration in plant tissue the predicted contribution of soil or bedrock extracts instead of that of total soil or bedrock concentrations.</style></abstract><issue><style face="normal" font="default" size="100%">1-3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 16648953</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">De Nicola, Flavia</style></author><author><style face="normal" font="default" size="100%">Maisto, Giulia</style></author><author><style face="normal" font="default" size="100%">Prati, Maria Vittoria</style></author><author><style face="normal" font="default" size="100%">Alfani, Anna</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Temporal variations in PAH concentrations in Quercus ilex L. (holm oak) leaves in an urban area.</style></title><secondary-title><style face="normal" font="default" size="100%">Chemosphere</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Aromatic</style></keyword><keyword><style  face="normal" font="default" size="100%">Aromatic: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cities</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Monitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Pollutants</style></keyword><keyword><style  face="normal" font="default" size="100%">Environmental Pollutants: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Italy</style></keyword><keyword><style  face="normal" font="default" size="100%">Mediterranean evergreen oak</style></keyword><keyword><style  face="normal" font="default" size="100%">Naples (Italy)</style></keyword><keyword><style  face="normal" font="default" size="100%">PAH biomonitoring</style></keyword><keyword><style  face="normal" font="default" size="100%">pah leaf accumulation</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Polycyclic Hydrocarbons</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: chemistry</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasonal trend</style></keyword><keyword><style  face="normal" font="default" size="100%">Seasons</style></keyword><keyword><style  face="normal" font="default" size="100%">Time Factors</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2005</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2005///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16182861</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">61</style></volume><pages><style face="normal" font="default" size="100%">432 - 440</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Temporal variations of polycyclic aromatic hydrocarbon (PAH) concentrations in leaves of a Mediterranean evergreen oak, Quercus ilex L., were investigated in order to assess the suitability of this species to biomonitor PAH air contamination. Leaf samples were collected at six sites of the urban area of Naples (Italy) and at a control site in the Vesuvius National Park, in May and September 2001, and in January and May 2002. PAH extraction was conducted by sonication in dichloromethane-acetone and quantification by GC-MS. In winter, leaf total PAH concentrations showed, at all the urban sites, values 2-fold higher than in all the other samplings, reflecting the temporal trend reported for PAH air contamination in the Naples urban area. Moreover, leaf PAH concentrations showed, at all the urban sites, a decrease in May 2002 after the winter accumulation. At the control site leaf PAH concentrations showed lower values and smaller temporal variations than at the urban sites. The findings support the suitability of Q. ilex leaves to monitor temporal variations in PAH contamination. The highest winter concentrations of total PAHs were due to the medium molecular weight PAHs that increased with respect to both low and high molecular weight PAHs. The medium molecular weight PAHs showed the same temporal trend both at the urban and remote sites.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 16182861</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Criquet, Stéven</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Measurement and characterization of cellulase activity in sclerophyllous forest litter.</style></title><secondary-title><style face="normal" font="default" size="100%">Journal of microbiological methods</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Buffers</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulase</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulase: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulase: isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose</style></keyword><keyword><style  face="normal" font="default" size="100%">Cellulose: metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Endoglucanase</style></keyword><keyword><style  face="normal" font="default" size="100%">Extraction</style></keyword><keyword><style  face="normal" font="default" size="100%">Hydrogen-Ion Concentration</style></keyword><keyword><style  face="normal" font="default" size="100%">Isoenzyme</style></keyword><keyword><style  face="normal" font="default" size="100%">Isoenzymes</style></keyword><keyword><style  face="normal" font="default" size="100%">Isoenzymes: analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">litter</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Leaves: enzymology</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus: enzymology</style></keyword><keyword><style  face="normal" font="default" size="100%">Temperature</style></keyword><keyword><style  face="normal" font="default" size="100%">Trees</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2002///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/11997167</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">50</style></volume><pages><style face="normal" font="default" size="100%">165 - 173</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Cellulases are enzymatic proteins which hydrolyze cellulose polymers to smaller oligosaccharides, cellobiose and glucose. They consist in three major types of enzymes: endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) and beta-glucosidases (EC 3.2.1.21) which play an essential role in carbon turnover of forest ecosystem. The aim of this study was firstly to determine the parameters (i.e. buffer type, pH, temperature, quantity of litter, incubation time and reagent type) which affect the measurement of cellulase activity in a sclerophyllous forest litter, and secondly to compare two methods for measuring cellulase activity: a direct method and an extraction method. In the direct method, the litter was directly incubated with a buffered solution containing the enzyme substrate, whereas in the extraction method, the cellulases were firstly extracted before measuring their activity. The results were compared with other studies about soil cellulase activity, and it appeared that several parameters (buffer type, pH, temperature and sample quantity) which influence the measurement of cellulase activity differ according to whether a soil or a litter is considered. Concerning the procedure used for the measurement of cellulase activity, results showed that the activity values were higher when using an extraction procedure than when using a direct procedure. The extraction procedure, combined with a concentration stage of the extract, also allowed electrophoretic analysis (PAGE) of the cellulases extracted from the litter. The electrophoretic pattern revealed two cellulase isoenzymes which may be related to the occurrence of two pH-activity peaks of these enzymes when citrate buffer was used for the measurement of cellulase activity in the litter.</style></abstract><issue><style face="normal" font="default" size="100%">2</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;accession-num: 11997167</style></notes></record></records></xml>