<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Romano, Anabela</style></author><author><style face="normal" font="default" size="100%">Martins-Loução, M a</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In vitro cold storage of cork oak shoot cultures</style></title><secondary-title><style face="normal" font="default" size="100%">Plant cell, tissue and organ culture</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">in vitro conservation</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus suber</style></keyword><keyword><style  face="normal" font="default" size="100%">shoot proliferation</style></keyword><keyword><style  face="normal" font="default" size="100%">woody species</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">1999</style></year></dates><volume><style face="normal" font="default" size="100%">59</style></volume><pages><style face="normal" font="default" size="100%">155-157</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">A simple system for in vitro conservation of cork oak shoot cultures (Quercus suber L.) is described. Cultures were stored in vitro on multiplication medium at 51 C without an intervening subculture for two years. The viability, multiplication rate and shoot elongation were evaluated after storage under dark and light conditions. Culture viability was negatively affected by light. In contrast, 50% of cultures survived after two years of cold storage in the dark. Multiplication rate of dark-stored cultures was similar to the controls, and shoot elongation was signiﬁcantly higher. Although the assessment of multiplication rate/shoot elongation is done at the end of the ﬁrst multiplication cycle, we observed that at least in some species such as Quercus suber, it is advisable to study the responsiveness of cultures during the ﬁrst three multiplication cycles following storage. The rooting capacity of shoots produced from dark-stored cultures was similar to non-stored controls. Cultures stored for 6, 12 or 24 months without subculture have similar responsiveness as the ones subcultured monthly. The storage of cultures at 5 C in the dark appears to be a promising technique for medium-term conservation of cork oak germplasm.</style></abstract></record></records></xml>