Home /Cryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors.

Cryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors.

TitleCryopreservation of Quercus suber and Quercus ilex embryonic axes: in vitro culture, desiccation and cooling factors.
Publication TypeJournal Article
Year of Publication2002
AuthorsM Gonzalez-Benito, E., Prieto R-M., Herradon E., & Martin C.
JournalCryo letters
Volume23
Pagination283-290
Accession Number12447487
KeywordsCryopreservation, Cryopreservation: methods, Cryoprotective Agents, Cryoprotective Agents: pharmacology, Culture Techniques, Culture Techniques: methods, Desiccation, Desiccation: methods, embryonic axes, germination, germination temperature, Germination: drug effects, liquid nitrogen, plantlet development, Quercus, Quercus: embryology, Seeds, Seeds: chemistry, Seeds: embryology, Survival Rate, Temperature, water, Water: analysis
Abstract

This study examines different factors included in the cryopreservation protocols for Quercus ilex and Q. suber embryonic axes. In vitro incubation temperature played an important role in the appropriate development of Q. ilex axes, as 15 degrees C was superior to 25 degrees C. Q. suber axes proved to be more sensitive to desiccation and cooling. Poor survival (35%) was observed when axes were included into cryovials and then in liquid nitrogen, and none when immersed in sub-cooled liquid nitrogen (-210 degrees C). Q. ilex axes showed poorly organised development in vitro (c. 50% of non-cooled axes showed shoot development). However, c. 80% survival was observed after cryopreservation (either in liquid nitrogen or sub-cooled liquid nitrogen at 0.34 g water / g dry weight), of which c. 15% showed shoot development.