Manganese depletion in chloroplasts of Quercus rotundifolia during chemical simulation of lichen epiphytic states

Incubation of Quercus rotundifolia branches in culture media containing lichen substances isolated from Evernia prunastri thallus produces inhibition of Hill reaction in chloroptasts isolated from the leaves of the branches. Incubation with lichen substances diminishes the amount of chloroplastic manganese through a chelation process. This decrease causes the loss of photosynthetic activity because the Hill reaction is restored upon addition of exogenous manganese to the chloropiasts.