<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fischbach, Robert Josef</style></author><author><style face="normal" font="default" size="100%">Staudt, Michael</style></author><author><style face="normal" font="default" size="100%">Zimmer, Ina</style></author><author><style face="normal" font="default" size="100%">Rambal, Serge</style></author><author><style face="normal" font="default" size="100%">Schnitzler, Jörg-Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Seasonal pattern of monoterpene synthase activities in leaves of the evergreen tree Quercus ilex</style></title><secondary-title><style face="normal" font="default" size="100%">Physiologia Plantarum</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">monoterpene emissions</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene synthase activities</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword><keyword><style  face="normal" font="default" size="100%">seasonal patterns (voyant)</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2002</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2002///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1034/j.1399-3054.2002.1140304.xhttp://www.ncbi.nlm.nih.gov/pubmed/12060257</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">114</style></volume><pages><style face="normal" font="default" size="100%">354 - 360</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Monoterpene synthase activities were measured in current year and 1-year-old leaves of holm oak (Quercus ilex L.). The monoterpene synthase activities of the leaves strongly changed with leaf development and leaf age. Enzyme activities increased rapidly in spring after leaf emergence, reaching maximum values in summer, which declined during the following winter period. In the next spring monoterpene synthase activities recovered in the old leaves to about one-third of values in the previous years and showed a similar seasonal variation as in young leaves. In both leaf age classes the pattern of enzymatic monoterpene formation was stable with α-pinene (33%), β-pinene (28%), and myrcene (26%) as prominent compounds followed by minor fractions of sabinene (10%) and limonene (3%). Monoterpene emission correlated with the activity of the synthetizing enzymes, indicating that monoterpene synthase activities in Q. ilex reflect the seasonal monoterpene emission potential of the leaves.</style></abstract><issue><style face="normal" font="default" size="100%">3</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Munksgaard International Publishers&lt;br/&gt;accession-num: 12060257</style></notes></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Seasonal pattern of monoterpene synthase activities in leaves of the evergreen tree Quercus ilex</style></title><secondary-title><style face="normal" font="default" size="100%">Physiologia Plantarum</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2002</style></year></dates><publisher><style face="normal" font="default" size="100%">Munksgaard International Publishers</style></publisher><volume><style face="normal" font="default" size="100%">114</style></volume><pages><style face="normal" font="default" size="100%">354-360</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Monoterpene synthase activities were measured in current year and 1-year-old leaves of holm oak (Quercus ilex L.). The monoterpene synthase activities of the leaves strongly changed with leaf development and leaf age. Enzyme activities increased rapidly in spring after leaf emergence, reaching maximum values in summer, which declined during the following winter period. In the next spring monoterpene synthase activities recovered in the old leaves to about one-third of values in the previous years and showed a similar seasonal variation as in young leaves. In both leaf age classes the pattern of enzymatic monoterpene formation was stable with α-pinene (33%), β-pinene (28%), and myrcene (26%) as prominent compounds followed by minor fractions of sabinene (10%) and limonene (3%). Monoterpene emission correlated with the activity of the synthetizing enzymes, indicating that monoterpene synthase activities in Q. ilex reflect the seasonal monoterpene emission potential of the leaves.</style></abstract><accession-num><style face="normal" font="default" size="100%">12060257</style></accession-num></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation and functional analysis of a cDNA encoding a myrcene synthase from holm oak (Quercus ilex L.)</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Biochemistry</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2001</style></year></dates><publisher><style face="normal" font="default" size="100%">Blackwell Science Ltd</style></publisher><volume><style face="normal" font="default" size="100%">268</style></volume><pages><style face="normal" font="default" size="100%">5633-5638</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An 859-bp cDNA segment of a terpene synthase gene was amplified by PCR from the evergreen sclerophyllous holm oak (Quercus ilex L.) using heterologous primers for conserved regions of terpene synthase genes (TPS) in dicotyledonous plants. Based on the sequence of this segment, homologous primers were designed for amplification by RACE-PCR of a cDNA segment carrying the monoterpene synthase gene myrS. The gene encodes a protein of 597 amino acids including an N-terminal putative plastid transit peptide. The gene without the segment encoding the transit peptide was cloned by PCR into a bacterial expression vector. Expression in Escherichia coli yielded an active monoterpene synthase, which converted geranyl diphosphate (GDP) predominantly into the acyclic monoterpene myrcene and to a very small extent into cyclic monoterpenes. Sequence comparison with previously cloned monoterpene synthases revealed that the myrcene synthase from Q. ilex belongs to the TPSb subfamily.</style></abstract></record><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fischbach, Robert J.</style></author><author><style face="normal" font="default" size="100%">Zimmer, Wolfgang</style></author><author><style face="normal" font="default" size="100%">Schnitzler, Jörg-Peter</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Isolation and functional analysis of a cDNA encoding a myrcene synthase from holm oak (Quercus ilex L.)</style></title><secondary-title><style face="normal" font="default" size="100%">European Journal of Biochemistry</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">functional expression</style></keyword><keyword><style  face="normal" font="default" size="100%">geranyl diphosphate</style></keyword><keyword><style  face="normal" font="default" size="100%">monoterpene synthases</style></keyword><keyword><style  face="normal" font="default" size="100%">myrcene synthase</style></keyword><keyword><style  face="normal" font="default" size="100%">Quercus ilex</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2001</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2001///</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://dx.doi.org/10.1046/j.1432-1033.2001.02519.x</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">268</style></volume><pages><style face="normal" font="default" size="100%">5633 - 5638</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">An 859-bp cDNA segment of a terpene synthase gene was amplified by PCR from the evergreen sclerophyllous holm oak (Quercus ilex L.) using heterologous primers for conserved regions of terpene synthase genes (TPS) in dicotyledonous plants. Based on the sequence of this segment, homologous primers were designed for amplification by RACE-PCR of a cDNA segment carrying the monoterpene synthase gene myrS. The gene encodes a protein of 597 amino acids including an N-terminal putative plastid transit peptide. The gene without the segment encoding the transit peptide was cloned by PCR into a bacterial expression vector. Expression in Escherichia coli yielded an active monoterpene synthase, which converted geranyl diphosphate (GDP) predominantly into the acyclic monoterpene myrcene and to a very small extent into cyclic monoterpenes. Sequence comparison with previously cloned monoterpene synthases revealed that the myrcene synthase from Q. ilex belongs to the TPSb subfamily.</style></abstract><issue><style face="normal" font="default" size="100%">21</style></issue><notes><style face="normal" font="default" size="100%">The following values have no corresponding Zotero field:&lt;br/&gt;publisher: Blackwell Science Ltd</style></notes></record></records></xml>