Micropropagation of cork oak (Quercus suber L.) by apical bud culture from embryonic axes

To improve the in vitro germination and development of cork Oakembryonic axes, several mineral media were tested; Durzan mediummodified 1/2 supplemented with 400 mg/l glutamine and 3% sucrose gavethe best results. The action of BA alone or combined with other growthregulators was also studied. A low concentration (0.44 mu M) was foundto give an acceptable percentage of plantlets. When combined with NAA,callogenesis was observed. The transfer of cultures in conditions of lowluminosity led to the formation of several axillary buds. These budswere multiplied on a solid medium supplemented with GA(3) at lowconcentration. The buds were elongated, then rooted on a growthregulator-free medium after brief dipping in an auxinic solution. Therooted plants were potted on horticultural substrate.