Micropropagation of cork oak (Quercus suber L.) by apical bud culture from embryonic axes

To improve the in vitro germination and development of cork Oakembryonic axes, several mineral media were tested; Durzan medium
modified 1/2 supplemented with 400 mg/l glutamine and 3% sucrose gave
the best results. The action of BA alone or combined with other growth
regulators was also studied. A low concentration (0.44 mu M) was found
to give an acceptable percentage of plantlets. When combined with NAA,
callogenesis was observed. The transfer of cultures in conditions of low
luminosity led to the formation of several axillary buds. These buds
were multiplied on a solid medium supplemented with GA(3) at low
concentration. The buds were elongated, then rooted on a growth
regulator-free medium after brief dipping in an auxinic solution. The
rooted plants were potted on horticultural substrate.