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Genetic transformation of cork oak (Quercus suber L.) for herbicide resistance.

TitleGenetic transformation of cork oak (Quercus suber L.) for herbicide resistance.
Publication TypeJournal Article
Year of Publication2009
AuthorsAlvarez, R., Alvarez J. M., Humara J. M., Revilla A., & Ordás R. J.
JournalBiotechnology letters
Volume31
Pagination1477-83
Accession Number19543858
KeywordsAcetyltransferases, Acetyltransferases: genetics, Acetyltransferases: metabolism, Gene Dosage, Genomic Instability, Herbicide Resistance, Herbicides, Herbicides: toxicity, Plant Proteins, Plant Proteins: genetics, Plant Proteins: metabolism, Plants, Genetically Modified, Plants, Genetically Modified: drug effects, Plants, Genetically Modified: genetics, Plants, Genetically Modified: physiology, Plasmids, Promoter Regions, Genetic, Quercus, Quercus: drug effects, Quercus: genetics, Quercus: physiology, Rhizobium, Rhizobium: genetics, Transformation, Genetic, Zea mays, Zea mays: genetics
Abstract

The bar gene was introduced into the cork oak genome. Cork oak embryogenic masses were transformed using the Agrobacterium strain AGL1 which carried the plasmid pBINUbiBar. This vector harbours the genes, nptII and bar, the latter under control of the maize ubiquitin promoter. The transgenic embryogenic lines were cryopreserved. Varying activities of phosphinothricin acetyl transferase were detected among the lines, which carried 1-4 copies of the insert. Molecular and biochemical assays confirmed the stability and expression of the transgenes 3 months after thawing the cultures. These results demonstrate genetic engineering of herbicide tolerance in Quercus spp.