Determination of Poly(ethylene glycol)-Binding to Browse Foliage, as an Assay of Tannin, by Near-Infrared Reflectance Spectroscopy
| Title | Determination of Poly(ethylene glycol)-Binding to Browse Foliage, as an Assay of Tannin, by Near-Infrared Reflectance Spectroscopy |
| Publication Type | Journal Article |
| Year of Publication | 2004 |
| Authors | Landau, S., Dvash L., Decandia M., Cabiddu A., Shapiro F., Molle G., & Silanikove N. |
| Journal | Journal of Agricultural and Food Chemistry |
| Volume | 52 |
| Pagination | 638-642 |
| Accession Number | 14759161 |
| Keywords | Animal Nutritional Physiological Phenomena, Animals, Carbon Radioisotopes, Fabaceae, Fabaceae: chemistry, Goats, Hydrolyzable Tannins, Hydrolyzable Tannins: analysis, Near-Infrared, NIRS, nutrition, pasture, poly(ethylene glycol), Polyethylene Glycols, Polyethylene Glycols: metabolism, Spectroscopy |
| Abstract | Nutritionists are interested in functional assays of tannins that do not require time-consuming and expensive extraction, such as the 14C-labeled poly(ethylene glycol) (PEG)-binding (PEG-b) assay. This paper reports the application of near-infrared (NIR) spectroscopy to determine the percentage of PEG binding, in place of the 14C-labeled PEG-b assay of tannin, in Mediterranean woodland vegetation. Calibration was done with 53 samples from 14 species and was validated on 25 samples from 10 species. PEG-b ranged between 1.4 and 20.7% in the samples. The calibration obtained by using the modified partial least-squares (MPLS) method, with all wavelengths in the 1100?2500 nm range combined, and the validation were reasonably linear (R?2 = 0.96 and 0.91, respectively). The accuracies, estimated from the standard errors of cross-validation and prediction, were ±1.6 and ±1.7% PEG-b, respectively. The NIRS-aided procedure proposed here can serve as an accurate, inexpensive, time-saving, and environment-friendly functional assay of tannin in Mediterranean browse. Keywords: NIRS; goats; nutrition; poly(ethylene glycol); pasture |
