Seasonal and annual changes in leaf δ13C in two co-occurring Mediterranean oaks: relations to leaf growth and drought progression

1. Changes of δ13C and its relation to leaf development, biochemical content and water stress were monitored over a 2 year period in two co-occurring Mediterranean oak species: the deciduous Quercus pubescens and the evergreen Quercus ilex. 2. The time course of leaf δ13C showed different patterns in the two species. Young Q. pubescens leaves had a high δ13C and a marked decrease occurred during leaf growth. In contrast, leaves at budburst and maturity did not differ significantly in the case of Q. ilex. We suggest that the difference between δ13C of young leaves was linked to differential use of reserves of carbon compounds in the two species. 3.δ13C values of mature leaves were negatively correlated with minimum seasonal values of predawn water potential, suggesting that a functional adjustment to water resources occurred. 4. There was a significant correlation between individual δ13C values for two successive years. This interannual dependence showed that δ13C rankings between trees were constant through time.