Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture
Title | Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture |
Publication Type | Journal Article |
Year of Publication | 2010 |
Authors | Pintos, B., Manzanera J. a, & M. Bueno A. |
Journal | Annals of Forest Science |
Volume | 67 |
Issue | 2 |
Date Published | 2010/// |
Keywords | activated charcoal, amino acids, Cork oak, gametic embryo, plantlet acclimation |
Abstract | • Development of both somatic and gametic embryogenesis has many applications in clonal forestry and genetic improvement, for instance as mass-propagation of genetically improved plants and production of pure lines through doubled-haploid plant regeneration from gametic embryos. • The goal of this work was to improve growth, maturation and plantlet regeneration of cork oak (Quercus suber L.) embryos from both somatic and gametic origin. • Activated charcoal promoted a significant increase in growth in terms of relative size and weight of both somatic and doubled-haploid embryos, as well as a more efficient control of secondary somatic embryogenesis during development. A significant interaction was also observed with amino acid nutrition. While some amino acids (i.e., glutamine, arginine or asparagine) did not show significant differences with the controls, a mixture of these three amino acids or gamma amino butyric acid stimulated embryo growth. The highest survival rate during acclimation of plantlets from both somatic and doubled-haploid origin was obtained when the embryos had been previously cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4 ◦ C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid. • We obtained more than 900 cork oak plantlets acclimated from several embryogenic lines, with a high survival rate, demonstrating that this methodology is applicable for large scale plantlet production. We also report the first regeneration of doubled-haploid plantlets in cork oak. |
URL | http://www.springerlink.com/openurl.asp?id=doi:10.1051/forest/2009098 |